2-amino-2-(hydroxymethyl)propane-1,3-diol

• ChemBase ID: 3404
• Molecular Formular: C4H11NO3
• Molecular Mass: 121.13504
• Monoisotopic Mass: 121.07389322
• SMILES: NC(CO)(CO)CO
• Canonical SMILES: OCC(CO)(CO)N
• InChI: InChI=1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChIKey: LENZDBCJOHFCAS-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: 2-amino-2-(hydroxymethyl)propane-1,3-diol
• IUPAC Traditional name: tris buffer; tris-base
• Synonyms: Tris(Hydroxymethyl)Aminomethane; 2-Amino-2-(hydroxymethyl)-1,3-propanediol; Tris-[hydroxymethyl]amino-methane; TRIS ULTRA PURE GRADE; Tris(hydroxymethyl)aminomethane; ATX Tris buffer; Trizma® base; Sigma 7-9®; Tromethamine; Trometamol; Trometamol; Tromethamine; Tris-[hydroxymethyl]aminomethane; 2-Amino-2-(hydroxymethyl)-1,3-propanediol; TRIS TECHNICAL GRADE; TRIS, ULTRA PURE; 2-amino-2-(hydroxymethyl)propane-1,3-diol; Tris(hydroxymethyl)aminomethane, ACS; 2-Amino-2-hydroxymethyl-1,3-propanediol; Trometamol, Tromethamine, Tris-[hydroxymethyl]amino-methane; TRIS; TRIS USP GRADE; TRIS; Tris base; Tris buffer; Trizma^TM; Trisamine; THAM; Tromethane; Trometamolum; Tris(hydroxymethyl)aminomethane; Trizma® base; 三(羟基甲基)氨基甲烷; 2-氨基-2-(羟甲基)-1,3-丙二醇; Tris 碱; 氨基丁三醇; Trizma® 碱; 缓血酸胺; 三(羟甲基)氨基甲烷, ACS; 三(羟甲基)氨基甲烷; 氨丁三醇; 三羟甲基氨基甲烷; Trizma® 碱

Database IDs

• CAS Number: 77-86-1
• EC Number: 201-064-4
• MDL Number: MFCD00004679
• Beilstein Number: 3535773; 741883
• Merck Index: 149772
• PubChem SID: 24899973; 160966844; 24845756; 24900061; 24899961; 24889887; 24855244; 24900399; 24889850; 24852982; 24900345; 24900405; 46506027; 24889825; 24900556; 24889854
• PubChem CID: 6503; 3777159
• CHEBI ID: 9754
• ATC CODE: B05BB03; B05XX02
• CHEMBL: 1200391
• Chemspider ID: 6257
• KEGG ID: D00396
• Unique Ingredient Identifier: 023C2WHX2V
• Wikipedia Title: Tris

CALCULATED PROPERTIES

JChem

• Acid pKa: 14.164156
• H Acceptors: 4
• H Donor: 4
• LogD (pH = 5.5): -5.6058097
• LogD (pH = 7.4): -4.264039
• Log P: -2.712229
• Molar Refractivity: 28.3555 cm^3
• Polarizability: 11.566923 Å^3
• Polar Surface Area: 86.71 Å^2
• Rotatable Bonds: 3
• Lipinski's Rule of Five: true

ALOGPS 2.1

• Log P: -2.54
• LOG S: 0.37
• Solubility (Water): 3.70e+02 g/l

PROPERTIES

Physical Property

• Solubility: : soluble40 % (w/w), clear, colorless; ~50 g/100 mL (25 °C) in water; H2O: soluble1 M at 20 °C, clear; H2O: soluble1 M, clear, colorless; H2O: soluble4 M at 20 °C, clear, colorless
• Apperance: Crystalline; white crystalline; White crystalline powder; white to off-white crystalline; white to very faintly yellow crystalline; yellow tan crystalline
• Melting Point: >175-176 °C (448-449 K); 167-172 °C(lit.); 168-171 °C; 168-172 °C; 168-172°C; 171-172 °C; 171-172°C
• Boiling Point: 219°C; 219-220 °C at 10 mmHg; 219-220 °C/10 mmHg(lit.); 219-220°C/10mm
• Flash Point: Non-flammable
• Density: 1.328g/cm^3; 1.353
• Vapor Pressure: Low at ambient temperatures.
• Absorption Wavelength: λ: 230 nm Amax: ≤0.6; λ: 260 nm Amax: ≤0.05; λ: 260 nm Amax: ≤0.10; λ: 260 nm Amax: 0.10; λ: 280 nm Amax: ≤0.04; λ: 280 nm Amax: ≤0.08; λ: 280 nm Amax: 0.08; λ: 430 nm Amax: ≤0.01
• pKa: 8.07; 8.1 (25°C)
• pH: 10.3-10.8 (0.1 M); 10.5-11.5 (25 °C, 10% in H2O); 10.5-12.0 (1 M in H2O); 10.5-12.0 (25 °C, 4 M in H2O)

Safety Information

• Storage Condition: Room Temperature (15-30°C); Room Temperature (15-30°C), Desiccate
• Storage Warning: Hygroscopic; IRRITANT
• RTECS: TY2900000
• European Hazard Symbols: Irritant (Xi)
• German water hazard class: 2
• Risk Statements: 36/37/38; R:36/37/38; R36 R37 R38
• Safety Statements: 26-36; 26-37; S:20-25-26-37/39; S26 S36
• TSCA Listed: false; 是
• GHS Pictograms: GHS07
• GHS Signal Word: Warning
• Main Hazard: Irritant
• NFPA704:
  

  2
• GHS Hazard statements: H315-H319; H315-H319-H335
• GHS Precautionary statements: P261-P305 + P351 + P338; P261-P305+P351+P338-P302+P352-P321-P405-P501A; P305 + P351 + P338
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves; Eyeshields, Gloves

Pharmacology Properties

• Target: Others

Product Information

• Purity: ≥99%; ≥99% (titration); ≥99.0%; ≥99.0% (T); ≥99.5%; ≥99.7% (T); ≥99.8%; ≥99.8% (T); ≥99.9%; ≥99.9% (titration); 96%; 98%; 99%; 99.0-99.5%; 99.8-100.1%; 99.8-100.1% (Assay, dried basis); 99.9%; 99.95%
• Grade: ACS reagent; Biotechnology Performance Certified; for luminescence; for molecular biology; JIS special grade; Ph Eur; puriss. p.a.; TECHNICAL; ULTRA PURE; ultrapure grade; USP
• Salt Data: Free Base
• Suitability: cell culture tested; in accordance for luminescence; meets EP, USP testing specifications; meets USP testing specifications
• Ignition Residue: ≤0.01% (as SO4); ≤0.01% (as SO4) (900°C); ≤0.05% (as SO4)
• Description: aminopeptidase substrate
• Impurities: ≤0.0005% heavy metals (as Pb); ≤0.005% Insoluble matter; ≤0.005% insolubles; ≤0.2% water (Karl Fischer); ≤0.5% water (Karl Fischer); ≤2% water; DNase, RNase, protease, none detected; DNases, none detected; Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; RNases, none detected
• Cation Traces: Al: ≤0.0005%; Al: ≤5 mg/kg; As: ≤0.0001%; As: ≤0.1 mg/kg; Ba: ≤0.0005%; Ba: ≤5 mg/kg; Bi: ≤0.0005%; Bi: ≤5 mg/kg; Ca: ≤0.001%; Ca: ≤10 mg/kg; Cd: ≤0.0005%; Cd: ≤5 mg/kg; Co: ≤0.0005%; Co: ≤5 mg/kg; Cr: ≤0.0005%; Cr: ≤5 mg/kg; Cu: ≤0.0005%; Cu: ≤5 mg/kg; Fe: ≤0.0005%; Fe: ≤5 mg/kg; Fe: ≤5 ppm; heavy metals (as Pb): ≤2 ppm; heavy metals (as Pb): ≤5 ppm; K: ≤0.005%; K: ≤50 mg/kg; Li: ≤0.0005%; Li: ≤5 mg/kg; Mg: ≤0.0005%; Mg: ≤5 mg/kg; Mn: ≤0.0005%; Mn: ≤5 mg/kg; Mo: ≤0.0005%; Mo: ≤5 mg/kg; Na: ≤0.005%; Na: ≤50 mg/kg; Ni: ≤0.0005%; Ni: ≤5 mg/kg; Pb: ≤0.0005%; Pb: ≤5 mg/kg; Sr: ≤0.0005%; Sr: ≤5 mg/kg; Zn: ≤0.0005%; Zn: ≤0.5 mg/kg; Zn: ≤5 mg/kg
• Antion Traces: chloride (Cl-): ≤0.005%; chloride (Cl-): ≤20 mg/kg; chloride (Cl-): ≤5 mg/kg; sulfate (SO42-): ≤0.005%; sulfate (SO42-): ≤5 mg/kg
• Loss on Drying: ≤0.5% loss on drying, 110 °C; ≤0.5% loss on drying, 110°C; ≤1% loss on drying, 110 °C; ≤2% loss on drying, 110 °C
• pH Range: 7 - 9; 7.0 - 9.2
• Quality Level: ELITE
• Quality: certified by BAM, according to ISO 17025
• λ: 10 % in H2O; 4 M in H2O
• Useful pH Range: 7 - 9
• Pharmacopeia: testing & handling conforms to Pharmacopeia
• Product Line: BioUltra; BioXtra
• abs.: / passes test; A1M/260, H2O ≤0.025; A1M/280, H2O ≤0.020; A40%/290 ≤0.05; A40%/290 ≤0.4
• Linear Formula: NH2C(CH2OH)3
• Empirical Formula (Hill Notation): C4H11NO3

DETAILS

MP Biomedicals - 02103133

Ultra Pure Grade
Purity: 99.95% min.
Buffering pH range 7.0-9.0
pKa at 25°C = 8.1
Excellent biochemical and biological buffer for all applications where ultra high purity is required. MP offers a complete selection of Biological Buffers including: BES, BICINE, BIS-TRIS, HEPES, MOPS, PIPES, And many more...

MP Biomedicals - 02195605

USP Grade
Purity: 99.95% min.
Buffering pH range 7.0-9.0
pKa at 25°C = 8.1
Excellent biochemical and biological buffer for all applications where ultra high purity is required.

MP Biomedicals - 02103132

Technical Grade
Purity: 96% min.
Buffering pH range 7.0-9.0
pKa at 25°C = 8.1
This grade of tris is suitable for use as a standard biological buffer in less critical applications where economy is an important consideration.

MP Biomedicals - 04819620

(Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane) Purity: 99.9% Heavy metals (as Pb): <5 ppm O.D. (260nm) of a 5% saturated solution: <0.1 pKa at 20°C = 8.1 Biological buffer reagent.

MP Biomedicals - 02152176

Purity: 99.0-99.5%
Buffering pH range 7.0-9.0 pKa at 25°C = 8.1
This grade of tris is excellent where purity and value are both important. It is superior to our technical grade tris and less expensive than out ultra pure material. This grade will suffice for most buffer applications.

MP Biomedicals - 04819638

(Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane) Purity: 99.9% Heavy metals (as Pb): <5 ppm O.D. (260nm) of a 5% saturated solution: <0.1 pKa at 20°C = 8.1 Biological buffer reagent.

MP Biomedicals - 04819623

(Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane) Purity: 99.9% Heavy metals (as Pb): <5 ppm O.D. (260nm) of a 5% saturated solution: <0.1 pKa at 20°C = 8.1 Biological buffer reagent.

MP Biomedicals - 02194557

(Trometamol, Tromethamine, Tris-[hydroxymethyl]amino- methane) Cell Culture Reagent Purity: 99.95% min. Buffering pH range 7.0-9.0 pKa at 25°C = 8.1 Excellent biochemical and biological buffer for all applications where ultra high purity is required.

MP Biomedicals - 02194855

(Trometamol, Tromethamine, Tris-[hydroxymethyl]amino- methane) Molecular Biology Reagent Purity: 99.95% min. Buffering pH range 7.0-9.0 pKa at 25°C = 8.1 Excellent biochemical and biological buffer for all molecular biology applications.

Sigma Aldrich - 93350

Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - T1410

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93286

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - T4661

Analysis Note
Not pyrogen tested. Certificates of analysis are available with lot specific information.
Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Packaging
Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - T6066

Application
Not tested for use in parenteral applications as specified in the EP monograph.
Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - T6687

Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.

Sigma Aldrich - 93362

Other Notes
Running buffer component in non-denaturing agarose gel electrophoresis1; In non-denaturing and urea-denaturing polyacrylamide gel electrophoresis2; DNA digest analysis with capillary electrophoresis3.
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93304

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 30-5025

Suitability
for biological purposes

Sigma Aldrich - T1378

General description
A 40% (w/w) solution in water is clear, colorless to faint yellow
Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Sigma 7-9 is a registered trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 08656

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 33742

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 252859

Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
Packaging
100, 500 g in poly bottle

Sigma Aldrich - T87602

Packaging
1 kg in poly bottle
250 g in poly bottle
3 kg in poly drum

Sigma Aldrich - 93349

Other Notes
In a high molar concentration for molecular weight determination of peptides and proteins by gel electrophoresis1; Electrophoresis of proteins on cellulose acetate membranes2; Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis3; Determination of trace amounts of fluorine from a single sodium carbonate fusion of small geological sample masses4.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - T6791

Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 04577

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93440

Analysis Note
Exact concentration, expiry date, traceability and detailed information on certification can be found on the certificate and certification report, included in each package. Content and expiry date on label.
Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 154563

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Buffer and primary pH standard. pKa=8.3 at 20°C.1 Auxiliary material in pharmaceutical science.2,3
Packaging
1 kg in poly bottle
5 g in glass bottle
100, 500 g in poly bottle
5 kg in poly drum

Sigma Aldrich - 30-5020

Suitability
for electrophoresis

Sigma Aldrich - 21685

Packaging
6 bottles for the preparation of 1 L ready-to-use solution (each)

Sigma Aldrich - T1503

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
General description
2222A 40% (w/w) solution in water is clear and colorless.
Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Packaging
1 kg in poly bottle
25, 100, 250, 500 g in poly bottle
5, 10, 25, 50 kg in poly drum
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Protocols & Applications
Ensure correct concentrations of buffer solutions with the Buffer Calculator

DrugBank - DB03754

• Drug Groups: experimental
• Description: An organic amine proton acceptor. It is used in the synthesis of surface-active agents and pharmaceuticals; as an emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, as a biological buffer, and used as an alkalizer. (From Merck, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p1424)

REFERENCES

• Widely used biological buffer.