Trizma® base

• Catalog No.: 08656
• CAS Number: 77-86-1
• M. F.: C4H11NO3
• M. W.: 121.13504
• Purity: ≥99.8% (T)

SYNONYMS

• Title: Trizma® 碱
• IUPAC name: 2-amino-2-(hydroxymethyl)propane-1,3-diol
• IUPAC Traditional name: tris buffer
• Synonyms: 氨基丁三醇; 2-Amino-2-(hydroxymethyl)-1,3-propanediol; Tris base; Tris(hydroxymethyl)aminomethane; Trometamol; 2-氨基-2-(羟甲基)-1,3-丙二醇; Tris 碱; THAM; 三羟甲基氨基甲烷

DATABASE IDS

• CAS Number: 77-86-1
• EC Number: 201-064-4
• Beilstein Number: 741883
• MDL Number: MFCD00004679

PROPERTIES

• Ignition Residue: ≤0.01% (as SO4)
• Impurities: insoluble matter, passes filter test
• λ: 4 M in H2O
• Linear Formula: NH2C(CH2OH)3
• Loss on Drying: ≤0.5% loss on drying, 110°C
• Product Line: BioUltra
• Purity: ≥99.8% (T)
• Useful pH Range: 7 - 9
• Antion Traces: chloride (Cl-): ≤5 mg/kg
• Antion Traces: sulfate (SO42-): ≤5 mg/kg
• Cation Traces: Al: ≤5 mg/kg
• Cation Traces: As: ≤0.1 mg/kg
• Cation Traces: Ba: ≤5 mg/kg
• Cation Traces: Bi: ≤5 mg/kg
• Cation Traces: Ca: ≤10 mg/kg
• Cation Traces: Cd: ≤5 mg/kg
• Cation Traces: Co: ≤5 mg/kg
• Cation Traces: Cr: ≤5 mg/kg
• Cation Traces: Cu: ≤5 mg/kg
• Cation Traces: Fe: ≤5 mg/kg
• Cation Traces: K: ≤50 mg/kg
• Cation Traces: Li: ≤5 mg/kg
• Cation Traces: Mg: ≤5 mg/kg
• Cation Traces: Mn: ≤5 mg/kg
• Cation Traces: Mo: ≤5 mg/kg
• Cation Traces: Na: ≤50 mg/kg
• Cation Traces: Ni: ≤5 mg/kg
• Cation Traces: Pb: ≤5 mg/kg
• Cation Traces: Sr: ≤5 mg/kg
• Cation Traces: Zn: ≤5 mg/kg
• Description: aminopeptidase substrate
• Absorption Wavelength: λ: 260 nm Amax: ≤0.10
• Absorption Wavelength: λ: 280 nm Amax: ≤0.08
• pKa: 8.1 (25°C)
• Boiling Point: 219-220 °C/10 mmHg(lit.)
• Melting Point: 167-172 °C(lit.)
• Melting Point: 168-172 °C
• pH: 10.5-12.0 (25 °C, 4 M in H2O)
• Solubility: H2O: soluble4 M at 20 °C, clear, colorless
• GHS Signal Word: Warning
• GHS Hazard statements: H315-H319-H335
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• GHS Precautionary statements: P261-P305 + P351 + P338
• Risk Statements: 36/37/38
• RTECS: TY2900000
• Safety Statements: 26-36
• German water hazard class: 2

DETAILS

Description (English)

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Description (简体中文)

Other Notes
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC