2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)acetic acid; 2-amino-2-(hydroxymethyl)propane-1,3-diol; boric acid

• ChemBase ID: 153117
• Molecular Formular: C14H30BN3O14
• Molecular Mass: 475.2107
• Monoisotopic Mass: 475.18208306
• SMILES: B(O)(O)O.C(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.C(C(CO)(CO)N)O
• Canonical SMILES: OC(=O)CN(CC(=O)O)CCN(CC(=O)O)CC(=O)O.OB(O)O.OCC(CO)(CO)N
• InChI: InChI=1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
• InChIKey: OSBLTNPMIGYQGY-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: 2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)acetic acid; 2-amino-2-(hydroxymethyl)propane-1,3-diol; boric acid
• IUPAC Traditional name: boric acid; edta; tris buffer
• Synonyms: TBE buffer; Tris-Borate-EDTA buffer; TBE 缓冲液; Tris-硼酸-EDTA 缓冲液

Database IDs

• MDL Number: MFCD00236358
• PubChem SID: 162247257
• PubChem CID: 21257725

CALCULATED PROPERTIES

• Acid pKa: 1.486185
• H Acceptors: 10
• H Donor: 4
• LogD (pH = 5.5): -10.586659
• LogD (pH = 7.4): -14.721412
• Log P: -5.221795
• Molar Refractivity: 62.3456 cm^3
• Polarizability: 24.69443 Å^3
• Polar Surface Area: 155.68 Å^2
• Rotatable Bonds: 14
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Apperance: powder blend; working solution

Safety Information

• European Hazard Symbols: Toxic (T)
• German water hazard class: 1; 2
• Risk Statements: 60-61; 60-61-36/37/38
• Safety Statements: 23-24/25; 53-26-45; 53-45
• GHS Pictograms: GHS07; GHS08
• GHS Signal Word: Danger
• GHS Hazard statements: H315-H319-H335-H360; H315-H319-H360; H360FD
• GHS Precautionary statements: P201-P261-P305 + P351 + P338-P308 + P313; P201-P305 + P351 + P338-P308 + P313; P201-P308 + P313
• Personal Protective Equipment: Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter; Eyeshields, Gloves, type P2 (EN 143) respirator cartridges

Product Information

• Grade: for molecular biology
• Suitability: suitable for electrophoresis; suitable for gel electrophoresis (after dilution to working concentration)
• Impurities: ≤5 ppm heavy metals (as Pb); bioburden, tested; DNase and RNase, none detected; DNase, RNase and NICKase, none detected; DNase, RNase, Protease, none detected; Dnase, RNase, Protease, tested; endotoxin, tested
• Sterility: 0.2 μm filtered; sterile; 0.2 μm filtered
• Quality Level: ELITE; GMP
• Product Line: BioReagent

DETAILS

Sigma Aldrich - T9525

Application
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis.1, TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.2 TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Other Notes
89 mM Tris borate, pH approx. 8.3, containing 2 mM EDTA.
Packaging
Supplied in dispenser with a spigot.
Preparation Note
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
Solution prepared with 18 megohm water

Sigma Aldrich - T4415

Application
Ready for use in gel electrophoresis after dilution to working concentrations.
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Other Notes
TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Packaging
The 4L, 10L and 20L sizes are supplied in dispenser with a spigot.
Preparation Note
Prepared with 18 megohm water
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Sigma Aldrich - T6400

Application
Ready for use in gel electrophoresis after dilution to working concentrations.
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Other Notes
0.445 M Tris borate, pH approx. 8.3, containing 0.01 M EDTA.
Packaging
Supplied in dispenser with a spigot.
Preparation Note
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
Solution prepared with 18 megohm water

Sigma Aldrich - T4448

Application
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis.1, TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.2 TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
General description
Made with WFI water; contains 0.445 M Tris-borate, 10 mM EDTA, at pH ~8.3.
Packaging
Supplied in a dispenser with a spigot
Preparation Note
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Sigma Aldrich - T7527

Application
Ready for use in gel electrophoresis after dilution to working concentrations.
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaging
The 1-liter size is packaged in a plastic bottle large enough to contain the 5× concentrate.
Preparation Note
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
Reconstitution
Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water.

Sigma Aldrich - T3913

Application
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis.1, TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.2 TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaging
Packaged in pouches
Preparation Note
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
Reconstitution
Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water. A suitable container must be supplied.

Sigma Aldrich - T4323

Application
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis.1, TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.2 TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Caution
TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Preparation Note
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).