Tris-Borate-EDTA buffer

• Catalog No.: T6400
• M. F.: C14H30BN3O14
• M. W.: 475.2107

SYNONYMS

• Title: Tris-硼酸-EDTA 缓冲液
• IUPAC name: 2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)acetic acid; 2-amino-2-(hydroxymethyl)propane-1,3-diol; boric acid
• IUPAC Traditional name: boric acid; edta; tris buffer
• Synonyms: TBE 缓冲液; TBE buffer

DATABASE IDS

• MDL Number: MFCD00236358

PROPERTIES

• Impurities: DNase and RNase, none detected
• Quality Level: ELITE
• Sterility: 0.2 μm filtered
• Personal Protective Equipment: Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
• German water hazard class: 2

DETAILS

Description (English)

Application
Ready for use in gel electrophoresis after dilution to working concentrations.
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Other Notes
0.445 M Tris borate, pH approx. 8.3, containing 0.01 M EDTA.
Packaging
Supplied in dispenser with a spigot.
Preparation Note
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
Solution prepared with 18 megohm water

Description (简体中文)

Application
TBE 电泳缓冲液是 DNA 和 RNA 聚丙烯酰胺凝胶电泳最常用的缓冲液。 TBE 与非变性或变性（7M 脲）凝胶一起使用。通常也用于 DNA 自动测序凝胶。TBE 也可用于琼脂糖凝胶，但不建议用于回收核酸所用的制备凝胶。 TBE 浓缩储存液稀释至 1× TBE 电泳缓冲液可得到含有 89mM Tris-硼酸和 2mM EDTA、pH 为 8.3 的缓冲液。还可将 5× 或 10× 储存液加到丙烯酰胺/甲叉双丙烯酰胺储存液以制备 PAGE 凝胶。为达到最大分辨率，建议外加电压低于 5V/cm（单位电极间的距离）。
稀释至工作浓度后用于凝胶电泳。
Other Notes
0.445M Tris 硼酸，pH 约为 8.3，含有 0.01M EDTA。
包装
用带龙头分液器包装。
Preparation Note
用 18 兆欧水制备的溶液
采用经生物技术性能认证的 Trizma 碱（产品代码 T6066）、分子生物学试剂硼酸（产品代码 B6768）和 EDTA 二钠盐（产品代码 E5134）制备。