Tris-Borate-EDTA buffer

• Catalog No.: T7527
• M. F.: C14H30BN3O14
• M. W.: 475.2107

SYNONYMS

• Title: Tris-硼酸-EDTA 缓冲液
• IUPAC name: 2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)acetic acid; 2-amino-2-(hydroxymethyl)propane-1,3-diol; boric acid
• IUPAC Traditional name: boric acid; edta; tris buffer
• Synonyms: TBE 缓冲液; TBE buffer

DATABASE IDS

• MDL Number: MFCD00236358

PROPERTIES

• Grade: for molecular biology
• Impurities: DNase and RNase, none detected
• Product Line: BioReagent
• Suitability: suitable for electrophoresis
• Apperance: powder blend
• GHS Signal Word: Danger
• GHS Hazard statements: H315-H319-H335-H360
• European Hazard Symbols: Toxic (T)
• Personal Protective Equipment: Eyeshields, Gloves, type P2 (EN 143) respirator cartridges
• GHS Precautionary statements: P201-P261-P305 + P351 + P338-P308 + P313
• Risk Statements: 60-61-36/37/38
• Safety Statements: 53-26-45
• German water hazard class: 2

DETAILS

Description (English)

Application
Ready for use in gel electrophoresis after dilution to working concentrations.
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaging
The 1-liter size is packaged in a plastic bottle large enough to contain the 5× concentrate.
Preparation Note
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
Reconstitution
Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water.

Description (简体中文)

Application
TBE 电泳缓冲液是 DNA 和 RNA 聚丙烯酰胺凝胶电泳最常用的缓冲液。 TBE 与非变性或变性（7M 脲）凝胶一起使用。通常也用于 DNA 自动测序凝胶。TBE 也可用于琼脂糖凝胶，但不建议用于回收核酸所用的制备凝胶。 TBE 浓缩储存液稀释至 1× TBE 电泳缓冲液可得到含有 89mM Tris-硼酸和 2mM EDTA、pH 为 8.3 的缓冲液。还可将 5× 或 10× 储存液加到丙烯酰胺/甲叉双丙烯酰胺储存液以制备 PAGE 凝胶。为达到最大分辨率，建议外加电压低于 5V/cm（单位电极间的距离）。
稀释至工作浓度后用于凝胶电泳。
包装
1L 容量包装在体积足以容纳 5× 浓缩液的塑料瓶中。
Preparation Note
采用经生物技术性能认证的 Trizma 碱（产品代码 T6066）、分子生物学试剂硼酸（产品代码 B6768）和 EDTA 二钠盐（产品代码 E5134）制备。
Reconstitution
溶解于指定量的水中得到 5× 浓缩液（0.445M Tris-硼酸、10mM EDTA，pH 为 8.3）。