Tris-Borate-EDTA buffer

• Catalog No.: T4323
• M. F.: C14H30BN3O14
• M. W.: 475.2107

SYNONYMS

• Title: Tris-硼酸-EDTA 缓冲液
• IUPAC name: 2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)acetic acid; 2-amino-2-(hydroxymethyl)propane-1,3-diol; boric acid
• IUPAC Traditional name: boric acid; edta; tris buffer
• Synonyms: TBE 缓冲液; TBE buffer

DATABASE IDS

• MDL Number: MFCD00236358

PROPERTIES

• European Hazard Symbols: Toxic (T)
• Personal Protective Equipment: Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
• GHS Precautionary statements: P201-P305 + P351 + P338-P308 + P313
• Risk Statements: 60-61
• Safety Statements: 53-45
• German water hazard class: 2
• GHS Signal Word: Danger
• GHS Hazard statements: H315-H319-H360
• Grade: for molecular biology
• Impurities: DNase, RNase, Protease, none detected
• Impurities: bioburden, tested
• Impurities: endotoxin, tested
• Impurities: ≤5 ppm heavy metals (as Pb)
• Product Line: BioReagent
• Quality Level: GMP
• Sterility: sterile; 0.2 μm filtered
• Suitability: suitable for electrophoresis

DETAILS

Description (English)

Application
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis.1, TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.2 TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Caution
TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.
Preparation Note
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Description (简体中文)

Application
TBE 电泳缓冲液是 DNA 和 RNA 聚丙烯酰胺凝胶电泳最常用的缓冲液。1, TBE 与非变性或变性（7M 脲）凝胶一起使用。通常也用于 DNA 自动测序凝胶。2TBE 也可用于琼脂糖凝胶，但不建议用于回收核酸所用的制备凝胶。 TBE 浓缩储存液稀释至 1× TBE 电泳缓冲液可得到含有 89mM Tris-硼酸和 2mM EDTA、pH 为 8.3 的缓冲液。还可将 5× 或 10× 储存液加到丙烯酰胺/甲叉双丙烯酰胺储存液以制备 PAGE 凝胶。为达到最大分辨率，建议外加电压低于 5V/cm（单位电极间的距离）。
Caution
TBE 缓冲剂会逐渐沉淀。通常而言，沉淀不会影响性能。
Preparation Note
采用经生物技术性能认证的 Trizma 碱（产品代码 T6066）、分子生物学试剂硼酸（产品代码 B6768）和 EDTA 二钠盐（产品代码 E5134）制备。