2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride

• ChemBase ID: 102812
• Molecular Formular: C4H12ClNO3
• Molecular Mass: 157.59598
• Monoisotopic Mass: 157.05057093
• SMILES: Cl.NC(CO)(CO)CO
• Canonical SMILES: OCC(CO)(CO)N.Cl
• InChI: InChI=1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChIKey: QKNYBSVHEMOAJP-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: 2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride
• IUPAC Traditional name: tris-base hydrochloride; tris buffer hydrochloride
• Synonyms: 2-Amino-2-hydroxymethyl-1,3-propanediol hydrochloride; Tris(hydroxymethyl)aminomethane hydrochloride; Trometamol; Tromethamine; Tris-[hydroxymethyl]aminomethane; TRIS HYDROCHLORIDE; TRIS HYDROCHLORIDE ULTRA PURE; 2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride; TRIS hydrochloride; Tris(hydroxymethyl)aminomethane hydrochloride; Tromethane hydrochloride; TRIS HCl; Trizma® hydrochloride; Tris Buffer substance; Tris(hydroxymethyl)aminomethane Buffer substance; Trizma® Buffer substance; Tromethamol, Tromethamine, Tris-[hydroxymethyl]aminomethane; Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane; Tris(hydroxymethyl)aminomethane hydrochloride buffer solution; Trizma® hydrochloride buffer solution; Tris hydrochloride solution; Trizma® hydrochloride solution; 2-AMino-2-(hydroxyMethyl)propane-1,3-diol hydrochloride; 三(羟甲基)氨基甲烷盐酸盐; 2-氨基-2-(羟甲基)-1,3-丙二醇 盐酸盐; 三甲醇氨基甲烷 盐酸盐; TRIS 盐酸盐; 三(羟甲基)氨基甲烷 盐酸盐; Trizma® 盐酸盐; Tris 缓冲物质; 三(羟甲基)氨基甲烷缓冲物质; Trizma® 缓冲物质; Tris(羟甲基)氨基甲烷盐酸盐缓冲溶液; Trizma® 盐酸盐缓冲溶液; Tris 盐酸盐溶液; Trizma® 盐酸盐 溶液

Database IDs

• CAS Number: 1185-53-1
• EC Number: 214-684-5
• MDL Number: MFCD00012590
• Beilstein Number: 3675235
• PubChem SID: 24889861; 24889826; 24889866; 24900398; 24889867; 24900037; 24900078; 24900332; 24900064; 162090647; 24889874; 24900044; 24889856; 24900102; 24866501; 24889860; 24889858; 24889833; 24889863; 24889868; 24889873; 24889865; 24889836; 24900093; 24889855; 24900119; 24889875; 24889834; 24866030
• PubChem CID: 93573

CALCULATED PROPERTIES

• Acid pKa: 14.164156
• H Acceptors: 4
• H Donor: 4
• LogD (pH = 5.5): -5.6058097
• LogD (pH = 7.4): -4.264039
• Log P: -2.712229
• Molar Refractivity: 28.3555 cm^3
• Polarizability: 11.566923 Å^3
• Polar Surface Area: 86.71 Å^2
• Rotatable Bonds: 3
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: : soluble40 % (w/w), clear, colorless; H2O: soluble, clear, colorless; H2O: soluble0.05 M at 20 °C, clear, faintly yellow; H2O: soluble0.5 M, clear, colorless; H2O: soluble0.67 g/mL, clear, colorless; H2O: soluble4 M at 20 °C, clear, colorless; H2O: soluble667 mg/mL
• Apperance: white crystalline
• Melting Point: 150-152 °C; 150-152°C (dec.); ca 152°C dec.
• Density: 1.05 g/mL at 20 °C
• Absorption Wavelength: A0.5M/260, H2O ≤0.015; A0.5M/280, H2O ≤0.010; A40%/290 ≤0.05; λ: 260 nm Amax: 0.03; λ: 260 nm Amax: 0.12; λ: 280 nm Amax: 0.04; λ: 280 nm Amax: 0.08
• pKa: 8.1; 8.1 (25°C)
• Fluorescence: λex 200-600 nm; λem 300-800 nm (corresponds)
• pH: 2.5-4.0 (25 °C, 4 M in H2O); 3.5-5.0 (0.5 M in H2O); 6.7±0.1 (37 °C, 0.05 M); 6.9±0.1 (37 °C, 0.05 M); 7.0; 7.0±0.1 (25 °C, 0.05 M); 7.1±0.1 (37 °C, 0.05 M); 7.2; 7.2±0.1 (25 °C); 7.2±0.1 (25 °C, 0.05 M); 7.2±0.1 (37 °C, 0.05 M); 7.3±0.1 (37 °C, 0.05 M); 7.4; 7.4±0.1 (25 °C); 7.4±0.1 (25 °C, 0.05 M); 7.4±0.1 (37 °C, 0.05 M); 7.5±0.1 (25 °C, 0.05 M); 7.52±0.05 (37 °C, 0.05 M); 7.6; 7.6±0.1; 7.6±0.1 (25 °C, 0.05 M); 7.6±0.1 (5 °C, 0.05 M); 7.7±0.1 (25 °C, 0.05 M); 7.7±0.1 (37 °C, 0.05 M); 7.8; 7.8±0.1 (37 °C, 0.05 M); 7.8±0.1 (5 °C, 0.05 M); 7.80±0.05 (25 °C, 0.05 M); 8.0; 8.0±0.1 (25 °C); 8.0±0.1 (25 °C, 0.05 M); 8.0±0.1 (37 °C, 0.05 M); 8.0±0.1 (5 °C, 0.05 M); 8.1±0.1 (25 °C, 0.05 M); 8.1±0.1 (5 °C, 0.05 M); 8.2±0.1 (5 °C, 0.05 M); 8.3±0.1 (25 °C, 0.05 M); 8.3±0.1 (5 °C, 0.05 M); 8.30±0.05 (25 °C, 0.05 m in H2O); 8.37±0.05 (5 °C, 0.05 M); 8.5±0.1 (37 °C, 0.05 M); 8.6±0.1 (5 °C, 0.05 M); 8.7±0.1 (5 °C, 0.05 M); 8.70±0.05 (37 °C, 0.05 M); 8.8±0.1 (25 °C, 0.05 M); 8.9±0.1 (5 °C, 0.05 M); 9.00±0.05 (25 °C, 0.05 M); 9.4±0.1 (5 °C, 0.05 M); 9.56±0.05 (5 °C, 0.05 M)

Safety Information

• Storage Condition: Room Temperature (15-30°C)
• Storage Warning: Hygroscopic
• European Hazard Symbols: Irritant (Xi)
• German water hazard class: 1; 2
• Risk Statements: 36/37/38; R:36/37/38
• Safety Statements: 26; S:20-25-26-37/39
• TSCA Listed: 是
• GHS Pictograms: GHS07
• GHS Signal Word: Warning
• GHS Hazard statements: H315-H319; H315-H319-H335
• GHS Precautionary statements: P261-P305 + P351 + P338; P305 + P351 + P338
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves; Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter; Eyeshields, Gloves; Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter

Product Information

• Purity: ≥99%; ≥99%; ≥99% (redox titration); ≥99.0% (AT); ≥99.0% (titration); 97%; 99%; 99+%
• Concentration: 1 M; 1.0 M tris-HCl
• Grade: Biotechnology Performance Certified; for fish-farming; for luminescence; for molecular biology; reagent grade
• Suitability: cell culture tested
• Ignition Residue: ≤0.2% (as SO4)
• Impurities: ≤0.5% water (Karl Fischer); DNase, RNase, NICKase and protease, none detected; DNase, RNase, protease, none detected; DNases, none detected; DNases, RNases, none detected; endotoxin and bioburden, tested; Insoluble matter, passes filter test; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; proteases, phosphatases, none detected; RNases, none detected
• Cation Traces: Al: ≤0.0005%; Al: ≤1 mg/kg; Al: ≤5 mg/kg; As: ≤0.0001%; As: ≤0.1 mg/kg; Ba: ≤0.0005%; Ba: ≤1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤0.0005%; Bi: ≤1 mg/kg; Bi: ≤5 mg/kg; Ca: ≤0.001%; Ca: ≤10 mg/kg; Ca: ≤5 mg/kg; Cd: ≤0.0005%; Cd: ≤1 mg/kg; Cd: ≤5 mg/kg; Co: ≤0.0005%; Co: ≤1 mg/kg; Co: ≤5 mg/kg; Cr: ≤0.0005%; Cr: ≤1 mg/kg; Cr: ≤5 mg/kg; Cu: ≤0.0005%; Cu: ≤1 mg/kg; Cu: ≤5 mg/kg; Fe: ≤0.0005%; Fe: ≤1 mg/kg; Fe: ≤5 mg/kg; heavy metals (as Pb): ≤0.0005%; heavy metals (as Pb): ≤5 ppm; K: ≤0.005%; K: ≤20 mg/kg; K: ≤50 mg/kg; Li: ≤0.0005%; Li: ≤1 mg/kg; Li: ≤5 mg/kg; Mg: ≤0.0005%; Mg: ≤1 mg/kg; Mg: ≤5 mg/kg; Mn: ≤0.0005%; Mn: ≤1 mg/kg; Mn: ≤5 mg/kg; Mo: ≤0.0005%; Mo: ≤1 mg/kg; Mo: ≤5 mg/kg; Na: ≤0.005%; Na: ≤20 mg/kg; Na: ≤50 mg/kg; Ni: ≤0.0005%; Ni: ≤1 mg/kg; Ni: ≤5 mg/kg; Pb: ≤0.0005%; Pb: ≤1 mg/kg; Pb: ≤5 mg/kg; Sr: ≤0.0005%; Sr: ≤1 mg/kg; Sr: ≤5 mg/kg; Zn: ≤0.0005%; Zn: ≤1 mg/kg; Zn: ≤5 mg/kg
• Antion Traces: sulfate (SO42-): ≤0.005%; sulfate (SO42-): ≤50 mg/kg
• Sterility: 0.2 μm filtered
• Loss on Drying: ≤0.2% loss on drying, 110 °C; ≤0.2% loss on drying, 110°C
• pH Range: 7.0 - 9.0
• Quality Level: ELITE; GMP
• Mol. Weight: average mol wt 124.6; average mol wt 127.2; average mol wt 135.4; average mol wt 139.8; average mol wt 141.8; average mol wt 145.8; average mol wt 147.6; average mol wt 149.0; average mol wt 150.6; average mol wt 151.6; average mol wt 153.8; average Mw 135.4; average Mw 154.8
• λ: 4 M in H2O; neat
• Useful pH Range: 7.0 - 9.0
• Product Line: BioReagent; BioUltra
• abs.: A40%/290 ≤0.05
• Linear Formula: NH2C(CH2OH)3 · HCl

DETAILS

MP Biomedicals - 02194856

(Tromethamol, Tromethamine, Tris-[hydroxymethyl]aminomethane) Molecular Biology Reagent Purity: >99% Hydrochloride

MP Biomedicals - 02103130

Hydrochloride
Crystalline
Purity: >99%

MP Biomedicals - 04816124

(Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane) Hydrochloride Purity: 99% Heavy metals as (Pb): <0.000022% RNAse and DNAse-free

MP Biomedicals - 02194558

Cell Culture Reagent
Hydrochloride
Crystalline
Purity: >99%

MP Biomedicals - 04816100

(Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane) Hydrochloride Purity: 99% Heavy metals as (Pb): <0.000022% RNAse and DNAse-free

MP Biomedicals - 04816116

(Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane) Hydrochloride Purity: 99% Heavy metals as (Pb): <0.000022% RNAse and DNAse-free

Sigma Aldrich - 93367

Application
water stabilizer for use in aquariums and for transporting tropical fish; stabilizes pH value and reduces CO2-level; suitable for marine species
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - T3253

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
所有缓冲液的 pH 值都与温度和浓度相关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
Other Notes
便于将 Trizma HCl 产品规格与 Trizma HCl 规格表进行比较。
包装
1 kg in poly bottle
100, 250, 500 g in poly bottle
5, 10, 25, 50 kg in poly drum
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - T2413

Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93369

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93394

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - T2538

Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93378

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93392

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - T5941

Application
所有缓冲液的 pH 值都与温度和浓度相关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
Other Notes
便于将 Trizma HCl 产品规格与 Trizma HCl 规格表进行比较。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - T3038

Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - T2663

Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - T2913

Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - T6666

Application
所有缓冲液的 pH 值都与温度和浓度相关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
Other Notes
便于将 Trizma HCl 产品规格与 Trizma HCl 规格表进行比较。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - T1535

Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93377

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93376

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93372

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 857645

Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 42861

Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93287

Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93371

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93379

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93313

Other Notes
Buffer component (SET buffer) in the preparation of RNA1
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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93374

Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - T2788

Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93316

Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93358

Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes
Standard buffer for use in the physiologic pH range1; Component of a cell-wash buffer that minimizes nucleic acid loss from Cl. perfringens 10543 A2; In chromatin preparation in the protein-free spreading technique for the electron microscopy of DNA and chromatin3.
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93314

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93363

Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes
Component of extraction buffer e.g. phenol extraction of DNA or RNA1; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE2.
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 93389

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Sigma Aldrich - 41573

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

REFERENCES

• Biological buffer.