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Trizma® hydrochloride

Catalog No. T5941 Name Sigma Aldrich
CAS Number 1185-53-1 Website http://www.sigmaaldrich.com
M. F. C4H12ClNO3 Telephone 1-800-521-8956
M. W. 157.59598 Fax
Purity ≥99.0% (titration) Email
Storage Chembase ID: 102812

SYNONYMS

Title
Trizma® 盐酸盐
IUPAC name
2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride
IUPAC Traditional name
tris buffer hydrochloride
Synonyms
TRIS HCl
三(羟甲基)氨基甲烷 盐酸盐
TRIS 盐酸盐

DATABASE IDS

PubChem SID 24900332
EC Number 214-684-5
CAS Number 1185-53-1
Beilstein Number 3675235
MDL Number MFCD00012590

PROPERTIES

Cation Traces heavy metals (as Pb): ≤5 ppm
Grade Biotechnology Performance Certified
Grade for molecular biology
Impurities DNase, RNase, protease, none detected
Impurities endotoxin and bioburden, tested
Impurities ≤0.5% water (Karl Fischer)
pH Range 7.0 - 9.0
Purity ≥99.0% (titration)
Quality Level ELITE
Suitability cell culture tested
Absorption Wavelength A40%/290 ≤0.05
pKa 8.1
Melting Point 150-152 °C
Solubility H2O: soluble667 mg/mL
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves
German water hazard class 1

DETAILS

Description (简体中文)
Application
所有缓冲液的 pH 值都与温度和浓度相关。对于 Tris 缓冲液,温度每降低 1°C,pH 增加约 0.03 个单位,每稀释 10 倍,pH 降低 0.03-0.05 个单位。 对于精确应用,使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
Other Notes
便于将 Trizma HCl 产品规格与 Trizma HCl 规格表进行比较。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

REFERENCES