Trizma® hydrochloride

• Catalog No.: 93363
• CAS Number: 1185-53-1
• M. F.: C4H12ClNO3
• M. W.: 157.59598
• Purity: ≥99.0% (AT)

SYNONYMS

• Title: Trizma® 盐酸盐
• IUPAC name: 2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride
• IUPAC Traditional name: tris buffer hydrochloride
• Synonyms: TRIS HCl; 三(羟甲基)氨基甲烷 盐酸盐; Tris(hydroxymethyl)aminomethane hydrochloride; TRIS hydrochloride; TRIS 盐酸盐

DATABASE IDS

• EC Number: 214-684-5
• CAS Number: 1185-53-1
• PubChem SID: 24889855
• Beilstein Number: 3675235
• MDL Number: MFCD00012590

PROPERTIES

• Antion Traces: sulfate (SO42-): ≤50 mg/kg
• Cation Traces: Al: ≤5 mg/kg
• Cation Traces: As: ≤0.1 mg/kg
• Cation Traces: Ba: ≤5 mg/kg
• Cation Traces: Bi: ≤5 mg/kg
• Cation Traces: Ca: ≤10 mg/kg
• Cation Traces: Cd: ≤5 mg/kg
• Cation Traces: Co: ≤5 mg/kg
• Cation Traces: Cr: ≤5 mg/kg
• Cation Traces: Cu: ≤5 mg/kg
• Cation Traces: Fe: ≤5 mg/kg
• Cation Traces: K: ≤50 mg/kg
• Cation Traces: Li: ≤5 mg/kg
• Cation Traces: Mg: ≤5 mg/kg
• Cation Traces: Mn: ≤5 mg/kg
• Cation Traces: Mo: ≤5 mg/kg
• Cation Traces: Na: ≤50 mg/kg
• Cation Traces: Ni: ≤5 mg/kg
• Cation Traces: Pb: ≤5 mg/kg
• Cation Traces: Sr: ≤5 mg/kg
• Cation Traces: Zn: ≤5 mg/kg
• Grade: for molecular biology
• Ignition Residue: ≤0.2% (as SO4)
• Impurities: DNases, none detected
• Impurities: RNases, none detected
• Impurities: insoluble matter, passes filter test
• Impurities: phosphatases, none detected
• Impurities: proteases, none detected
• λ: 4 M in H2O
• Linear Formula: NH2C(CH2OH)3 · HCl
• Loss on Drying: ≤0.2% loss on drying, 110 °C
• Product Line: BioUltra
• Purity: ≥99.0% (AT)
• Useful pH Range: 7.0 - 9.0
• Absorption Wavelength: λ: 260 nm Amax: 0.12
• Absorption Wavelength: λ: 280 nm Amax: 0.08
• pKa: 8.1 (25°C)
• pH: 2.5-4.0 (25 °C, 4 M in H2O)
• Solubility: H2O: soluble4 M at 20 °C, clear, colorless
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• German water hazard class: 1

DETAILS

Description (English)

Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes
Component of extraction buffer e.g. phenol extraction of DNA or RNA1; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE2.
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Description (简体中文)

Application
所有缓冲液的 pH 值都与温度和浓度相关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
Other Notes
Component of extraction buffer e.g. phenol extraction of DNA or RNA1; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE2.
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC