Trizma® hydrochloride

• Catalog No.: T1535
• CAS Number: 1185-53-1
• M. F.: C4H12ClNO3
• M. W.: 157.59598
• Purity: ≥99% (redox titration)

SYNONYMS

• Title: Trizma® 盐酸盐
• IUPAC name: 2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride
• IUPAC Traditional name: tris buffer hydrochloride
• Synonyms: TRIS HCl; 三(羟甲基)氨基甲烷 盐酸盐; Tris(hydroxymethyl)aminomethane hydrochloride; TRIS hydrochloride; TRIS 盐酸盐

DATABASE IDS

• EC Number: 214-684-5
• CAS Number: 1185-53-1
• Beilstein Number: 3675235
• MDL Number: MFCD00012590

PROPERTIES

• abs.: A40%/290 ≤0.05
• Cation Traces: heavy metals (as Pb): ≤0.0005%
• Impurities: ≤0.5% water (Karl Fischer)
• Linear Formula: NH2C(CH2OH)3 · HCl
• Purity: ≥99% (redox titration)
• Useful pH Range: 7.0 - 9.0
• pKa: 8.1 (25°C)
• Solubility: : soluble40 % (w/w), clear, colorless
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• German water hazard class: 1

DETAILS

Description (English)

Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Description (简体中文)

Application
所有缓冲液的 pH 值都与温度和浓度相关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC