2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid

• ChemBase ID: 102506
• Molecular Formular: C8H18N2O4S
• Molecular Mass: 238.30452
• Monoisotopic Mass: 238.09872807
• SMILES: OCCN1CCN(CC1)CCS(=O)(=O)O
• Canonical SMILES: OCCN1CCN(CC1)CCS(=O)(=O)O
• InChI: InChI=1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChIKey: JKMHFZQWWAIEOD-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
• IUPAC Traditional name: monosodium salt, hepes; hepes
• Synonyms: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; HEPES FREE ACID; HEPES solution; HEPES; HEPES buffer solution; 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid; HEPES buffered saline; 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid); HEPES 溶液; 4-(2-羟乙基)哌嗪-1-乙磺酸; N-(2-羟乙基)哌嗪-N′-(2-乙磺酸); HEPES 缓冲液; HEPES 缓冲盐溶液; 羟乙基哌嗪乙磺酸; HEPES

Database IDs

• CAS Number: 7365-45-9
• EC Number: 231-791-2; 230-907-9
• MDL Number: MFCD00006158
• Beilstein Number: 883043
• Merck Index: 144654
• PubChem SID: 24895616; 162088447; 24895796; 24895572; 24895414; 24878775; 24873833; 24888106; 24878774
• PubChem CID: 23831

CALCULATED PROPERTIES

• Acid pKa: -1.3356507
• H Acceptors: 6
• H Donor: 2
• LogD (pH = 5.5): -3.1127214
• LogD (pH = 7.4): -3.2495832
• Log P: -3.111727
• Molar Refractivity: 57.0168 cm^3
• Polarizability: 23.130201 Å^3
• Polar Surface Area: 81.08 Å^2
• Rotatable Bonds: 5
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: H2O: soluble1 M at 20 °C, clear, colorless; H2O: soluble1 M, clear, colorless
• Apperance: liquid
• Melting Point: 234°C; ca 236°C dec.
• Density: 1.07 g/mL at 20 °C
• Refractive Index: n20/D 1.339
• Absorption Wavelength: A0.1M/260, H2O ≤0.05; A0.1M/280, H2O ≤0.05; A1M/280, H2O ≤0.035; λ: 260 nm Amax: 0.045; λ: 260 nm Amax: 0.06; λ: 280 nm Amax: 0.035; λ: 280 nm Amax: 0.04
• pKa: 7.5; 7.5 (25°C)
• pH: 5.0-6.5 (1 M in H2O); 5.0-6.5 (25 °C, 1 M in H2O); 5.5±0.5; 7.0-7.6; 7.2 (25 °C)

Safety Information

• Storage Condition: Room Temperature (15-30°C), Desiccate
• RTECS: TL6809000
• German water hazard class: 1
• TSCA Listed: 是
• Personal Protective Equipment: Eyeshields, Gloves; Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US); Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: 2-8°C

Product Information

• Purity: ≥99.0%; ≥99.0% (T); ≥99.5% (T); ≥99.5% (titration); 98%; 99%
• Concentration: 1 M; 1 M in H2O; 238 g/L HEPES in H2O
• Grade: BioReagent; Biotechnology Performance Certified; for molecular biology
• Compostion: dextrose, 2.0 g/L; HEPES, 10 g/L; KCl, 0.74 g/L; Na2HPO4.2H2O, 0.27 g/L; NaCl, 16 g/L
• Suitability: cell culture tested; suitable for cell culture; suitable for molecular biology
• Ignition Residue: ≤0.1% (as SO4)
• Description: for research or for further manufacturing use; HEPES Solution 1M
• Impurities: Bioburden, tested; DNase, RNase, Protease, Nickase, free; DNases, none detected; endotoxin and total aerobic microbial count, tested; endotoxin, tested; Insoluble matter, passes filter test; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; RNases, none detected
• Cation Traces: Al: ≤1 mg/kg; Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤1 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Ca: ≤5 mg/kg; Ca: ≤50 mg/kg; Cd: ≤1 mg/kg; Cd: ≤5 mg/kg; Co: ≤1 mg/kg; Co: ≤5 mg/kg; Cr: ≤1 mg/kg; Cr: ≤5 mg/kg; Cu: ≤1 mg/kg; Cu: ≤5 mg/kg; Fe: <5 ppm; Fe: ≤1 mg/kg; Fe: ≤5 mg/kg; Fe: ≤5 ppm; heavy metals (as Pb): <5 ppm; heavy metals (as Pb): ≤5 ppm; K: ≤50 mg/kg; Li: ≤1 mg/kg; Li: ≤5 mg/kg; Mg: ≤1 mg/kg; Mg: ≤100 mg/kg; Mg: ≤5 mg/kg; Mn: ≤1 mg/kg; Mn: ≤5 mg/kg; Mo: ≤1 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤1 mg/kg; Ni: ≤5 mg/kg; Pb: ≤1 mg/kg; Pb: ≤5 mg/kg; Sr: ≤1 mg/kg; Sr: ≤5 mg/kg; Zn: ≤0.0005%; Zn: ≤1 mg/kg; Zn: ≤5 mg/kg
• Antion Traces: chloride (Cl-): ≤0.05%; chloride (Cl-): ≤50 mg/kg; sulfate (SO42-): ≤200 mg/kg; sulfate (SO42-): ≤300 mg/kg; sulfate (SO42-): ≤50 mg/kg
• Foreign Activity: DNase, RNase, NICKase, protease, none detected
• Sterility: 0.2 μm filtered; sterile-filtered
• Loss on Drying: ≤0.5% loss on drying, 110 °C; ≤1.0% loss on drying, 110°C
• pH Range: 6.8 - 8.2
• Quality Level: ELITE; GMP
• Quality: 2× concentrate
• λ: 1 M in H2O; neat
• Useful pH Range: 6.8 - 8.2
• Product Line: BioUltra
• Empirical Formula (Hill Notation): C8H18N2O4S

DETAILS

MP Biomedicals - 02101926

Crystalline
Free Acid
Zwitterionic Buffer useful in the pH range 6.8-8.2.
pKa=7.55 at 25°C

Sigma Aldrich - H3375

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Other Notes
使用 HEPES 规格表可以非常方便地比较 HEPES 产品的规格。
Protocols & Applications
Ensure correct concentrations of buffer solutions with the Buffer Calculator

Sigma Aldrich - H0887

Caution
Concentrations of HEPES over 40mM may be toxic to some cell types.
Preparation Note
Prepared from HEPES free acid and cell culture grade water.
Application
HEPES is an organic zwitterionic buffering agent effective in the physiological pH range of 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. This product has been qualified for use in cell culture.

Sigma Aldrich - 54457

Other Notes
3′-Terminal labeling of RNA with T4 RNA ligase1; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer2.
Easily compare specifications for HEPES products with the HEPES specification table.

Sigma Aldrich - 54459

Other Notes
One of the best general purpose buffers available for biological research. Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, CO2 fixation1,2; Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates3; Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials4.
Easily compare specifications for HEPES products with the HEPES specification table.

Sigma Aldrich - 59205C

Application
HEPES is used in many media because it has more buffering capacity in the physiologic pH range of 7.2 - 7.4 at 37 C than sodium bicarbonate. It is commonly added at concentrations between 10 mM and 25 mM. Higher levels cause cytotoxicity. Because bicarbonate is nutritionally necessary for most cells, HEPES should be added in addition to, not in place of, sodium bicarbonate.

Sigma Aldrich - H7523

Other Notes
使用 HEPES 规格表可以非常方便地比较 HEPES 产品的规格。

Sigma Aldrich - H4034

Other Notes
使用 HEPES 规格表可以非常方便地比较 HEPES 产品的规格。

Sigma Aldrich - 54461

Other Notes
Biological buffer1,2

Sigma Aldrich - 51558

Components
concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose

Sigma Aldrich - 13-2220

Suitability
for biological purposes

REFERENCES

• Biological buffer, pKa = 7.55 at 20^o: Biochemistry, 5, 467 (1966).