HEPES

• Catalog No.: 54457
• CAS Number: 7365-45-9
• M. F.: C8H18N2O4S
• M. W.: 238.30452
• Purity: ≥99.5% (T)

SYNONYMS

• IUPAC name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
• IUPAC Traditional name: hepes
• Synonyms: 4-(2-羟乙基)哌嗪-1-乙磺酸; N-(2-羟乙基)哌嗪-N′-(2-乙磺酸); N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid); 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

DATABASE IDS

• CAS Number: 7365-45-9
• Beilstein Number: 883043
• EC Number: 230-907-9
• MDL Number: MFCD00006158
• PubChem SID: 24878774

PROPERTIES

• Antion Traces: chloride (Cl-): ≤50 mg/kg
• Antion Traces: sulfate (SO42-): ≤50 mg/kg
• Cation Traces: Al: ≤5 mg/kg
• Cation Traces: As: ≤0.1 mg/kg
• Cation Traces: Ba: ≤5 mg/kg
• Cation Traces: Bi: ≤5 mg/kg
• Cation Traces: Ca: ≤10 mg/kg
• Cation Traces: Cd: ≤5 mg/kg
• Cation Traces: Co: ≤5 mg/kg
• Cation Traces: Cr: ≤5 mg/kg
• Cation Traces: Cu: ≤5 mg/kg
• Cation Traces: Fe: ≤5 mg/kg
• Cation Traces: K: ≤50 mg/kg
• Cation Traces: Li: ≤5 mg/kg
• Cation Traces: Mg: ≤5 mg/kg
• Cation Traces: Mn: ≤5 mg/kg
• Cation Traces: Mo: ≤5 mg/kg
• Cation Traces: Na: ≤50 mg/kg
• Cation Traces: Ni: ≤5 mg/kg
• Cation Traces: Pb: ≤5 mg/kg
• Cation Traces: Sr: ≤5 mg/kg
• Cation Traces: Zn: ≤5 mg/kg
• Empirical Formula (Hill Notation): C8H18N2O4S
• Grade: for molecular biology
• Ignition Residue: ≤0.1% (as SO4)
• Impurities: DNases, none detected
• Impurities: RNases, none detected
• Impurities: insoluble matter, passes filter test
• Impurities: phosphatases, none detected
• Impurities: proteases, none detected
• λ: 1 M in H2O
• Loss on Drying: ≤0.5% loss on drying, 110 °C
• Product Line: BioUltra
• Purity: ≥99.5% (T)
• Useful pH Range: 6.8 - 8.2
• Absorption Wavelength: λ: 260 nm Amax: 0.045
• Absorption Wavelength: λ: 280 nm Amax: 0.035
• pKa: 7.5 (25°C)
• pH: 5.0-6.5 (25 °C, 1 M in H2O)
• Solubility: H2O: soluble1 M at 20 °C, clear, colorless
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• RTECS: TL6809000
• German water hazard class: 1

DETAILS

Description (English)

Other Notes
3′-Terminal labeling of RNA with T4 RNA ligase1; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer2.
Easily compare specifications for HEPES products with the HEPES specification table.

Description (简体中文)

Other Notes
3′-Terminal labeling of RNA with T4 RNA ligase1; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer2.
Easily compare specifications for HEPES products with the HEPES specification table.