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HEPES

Catalog No. 54457 Name Sigma Aldrich
CAS Number 7365-45-9 Website http://www.sigmaaldrich.com
M. F. C8H18N2O4S Telephone 1-800-521-8956
M. W. 238.30452 Fax
Purity ≥99.5% (T) Email
Storage Chembase ID: 102506

SYNONYMS

IUPAC name
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
IUPAC Traditional name
hepes
Synonyms
4-(2-羟乙基)哌嗪-1-乙磺酸
N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

DATABASE IDS

CAS Number 7365-45-9
Beilstein Number 883043
EC Number 230-907-9
MDL Number MFCD00006158
PubChem SID 24878774

PROPERTIES

Antion Traces chloride (Cl-): ≤50 mg/kg
Antion Traces sulfate (SO42-): ≤50 mg/kg
Cation Traces Al: ≤5 mg/kg
Cation Traces As: ≤0.1 mg/kg
Cation Traces Ba: ≤5 mg/kg
Cation Traces Bi: ≤5 mg/kg
Cation Traces Ca: ≤10 mg/kg
Cation Traces Cd: ≤5 mg/kg
Cation Traces Co: ≤5 mg/kg
Cation Traces Cr: ≤5 mg/kg
Cation Traces Cu: ≤5 mg/kg
Cation Traces Fe: ≤5 mg/kg
Cation Traces K: ≤50 mg/kg
Cation Traces Li: ≤5 mg/kg
Cation Traces Mg: ≤5 mg/kg
Cation Traces Mn: ≤5 mg/kg
Cation Traces Mo: ≤5 mg/kg
Cation Traces Na: ≤50 mg/kg
Cation Traces Ni: ≤5 mg/kg
Cation Traces Pb: ≤5 mg/kg
Cation Traces Sr: ≤5 mg/kg
Cation Traces Zn: ≤5 mg/kg
Empirical Formula (Hill Notation) C8H18N2O4S
Grade for molecular biology
Ignition Residue ≤0.1% (as SO4)
Impurities DNases, none detected
Impurities RNases, none detected
Impurities insoluble matter, passes filter test
Impurities phosphatases, none detected
Impurities proteases, none detected
λ 1 M in H2O
Loss on Drying ≤0.5% loss on drying, 110 °C
Product Line BioUltra
Purity ≥99.5% (T)
Useful pH Range 6.8 - 8.2
Absorption Wavelength λ: 260 nm Amax: 0.045
Absorption Wavelength λ: 280 nm Amax: 0.035
pKa 7.5 (25°C)
pH 5.0-6.5 (25 °C, 1 M in H2O)
Solubility H2O: soluble1 M at 20 °C, clear, colorless
MSDS Link Download
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RTECS TL6809000
German water hazard class 1

DETAILS

Description (English)
Other Notes
3′-Terminal labeling of RNA with T4 RNA ligase1; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer2.
Easily compare specifications for HEPES products with the HEPES specification table.
Description (简体中文)
Other Notes
3′-Terminal labeling of RNA with T4 RNA ligase1; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer2.
Easily compare specifications for HEPES products with the HEPES specification table.

REFERENCES