HEPES solution

• Catalog No.: 59205C
• CAS Number: 7365-45-9
• M. F.: C8H18N2O4S
• M. W.: 238.30452

SYNONYMS

• Title: HEPES 溶液
• IUPAC name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
• IUPAC Traditional name: hepes
• Synonyms: N-(2-羟乙基)哌嗪-N′-(2-乙磺酸); N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

DATABASE IDS

• CAS Number: 7365-45-9
• MDL Number: MFCD00006158

PROPERTIES

• Description: HEPES Solution 1M
• Description: for research or for further manufacturing use
• Quality Level: GMP
• Useful pH Range: 6.8 - 8.2
• Apperance: liquid
• Storage Temperature: 2-8°C
• German water hazard class: 1

DETAILS

Description (English)

Application
HEPES is used in many media because it has more buffering capacity in the physiologic pH range of 7.2 - 7.4 at 37 C than sodium bicarbonate. It is commonly added at concentrations between 10 mM and 25 mM. Higher levels cause cytotoxicity. Because bicarbonate is nutritionally necessary for most cells, HEPES should be added in addition to, not in place of, sodium bicarbonate.

Description (简体中文)

Application
HEPES is used in many media because it has more buffering capacity in the physiologic pH range of 7.2 - 7.4 at 37 C than sodium bicarbonate. It is commonly added at concentrations between 10 mM and 25 mM. Higher levels cause cytotoxicity. Because bicarbonate is nutritionally necessary for most cells, HEPES should be added in addition to, not in place of, sodium bicarbonate.