HEPES

• Catalog No.: 54459
• CAS Number: 7365-45-9
• M. F.: C8H18N2O4S
• M. W.: 238.30452
• Purity: ≥99.5% (T)

SYNONYMS

• IUPAC name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
• IUPAC Traditional name: hepes
• Synonyms: 4-(2-羟乙基)哌嗪-1-乙磺酸; N-(2-羟乙基)哌嗪-N′-(2-乙磺酸); N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid); 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

DATABASE IDS

• CAS Number: 7365-45-9
• Beilstein Number: 883043
• PubChem SID: 24878775
• EC Number: 230-907-9
• MDL Number: MFCD00006158

PROPERTIES

• Antion Traces: chloride (Cl-): ≤50 mg/kg
• Antion Traces: sulfate (SO42-): ≤200 mg/kg
• Cation Traces: Al: ≤5 mg/kg
• Cation Traces: As: ≤0.1 mg/kg
• Cation Traces: Ba: ≤5 mg/kg
• Cation Traces: Bi: ≤5 mg/kg
• Cation Traces: Ca: ≤10 mg/kg
• Cation Traces: Cd: ≤5 mg/kg
• Cation Traces: Co: ≤5 mg/kg
• Cation Traces: Cr: ≤5 mg/kg
• Cation Traces: Cu: ≤5 mg/kg
• Cation Traces: Fe: ≤5 mg/kg
• Cation Traces: K: ≤50 mg/kg
• Cation Traces: Li: ≤5 mg/kg
• Cation Traces: Mg: ≤5 mg/kg
• Cation Traces: Mn: ≤5 mg/kg
• Cation Traces: Mo: ≤5 mg/kg
• Cation Traces: Na: ≤50 mg/kg
• Cation Traces: Ni: ≤5 mg/kg
• Cation Traces: Pb: ≤5 mg/kg
• Cation Traces: Sr: ≤5 mg/kg
• Cation Traces: Zn: ≤5 mg/kg
• Empirical Formula (Hill Notation): C8H18N2O4S
• Ignition Residue: ≤0.1% (as SO4)
• Impurities: insoluble matter, passes filter test
• λ: 1 M in H2O
• Loss on Drying: ≤0.5% loss on drying, 110 °C
• Product Line: BioUltra
• Purity: ≥99.5% (T)
• Useful pH Range: 6.8 - 8.2
• Absorption Wavelength: λ: 260 nm Amax: 0.045
• Absorption Wavelength: λ: 280 nm Amax: 0.035
• pKa: 7.5 (25°C)
• pH: 5.0-6.5 (25 °C, 1 M in H2O)
• Solubility: H2O: soluble1 M at 20 °C, clear, colorless
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• RTECS: TL6809000
• German water hazard class: 1

DETAILS

Description (English)

Other Notes
One of the best general purpose buffers available for biological research. Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, CO2 fixation1,2; Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates3; Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials4.
Easily compare specifications for HEPES products with the HEPES specification table.

Description (简体中文)

Other Notes
One of the best general purpose buffers available for biological research. Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, CO2 fixation1,2; Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates3; Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials4.
Easily compare specifications for HEPES products with the HEPES specification table.