Sigma Aldrich -
C9903
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Analysis Note
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.
Packaging
Package size based on protein content
Reconstitution
When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3 and 1 mM sodium EDTA.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic. |
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Sigma Aldrich -
C3741
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Application
Cholera toxin B subunit conjugated to peroxidase (CB-HRP) has been shown to be a more effective retrograde and transganglionic tracer for pelvic primary afferents from the urinary bladder than wheat germ agglutinin-horseradish peroxidase or isolectin B4-horseradish peroxidase. Retrograde labeling with CB-HRP has been demonstrated in symphathetic pre-ganglionic neurons as well as in cells of the fastigial nuclei. It is also an effective anterograde tracer for the study of axonal and terminal labeling in brain stem.
Caution
Do not freeze.
Packaging
Prepared and packaged using aseptic technique and sealed under vacuum.
Reconstitution
Reconstitution of the vial with 100 μl of water will yield a final solution containing: 1 mg/mL HRP, 0.45 mg/ml CTB, 10 mM phosphate buffer and 150 mM NaCl. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that may lead to foaming. Solutions can be sterile filtered through a 0.2 μm filter. Store the reconstituted solutions at 2-8 °C.
Specificity
HRP activity exceedes 100 pyrogallol units per mg of HRP in the CTB-HRP conjugate. Activity: 10-30 pyrogallol units per vial.
Unit Definition
Unit definition: One pyrogallol unit converts 1 mg of pyrogallol to purpurogallin in 20 seconds at pH 6.0 at 20 °C.
Physical form
Lyophilized powder containing 11-13% protein with the balance consisting of phosphate buffer and sodium chloride.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic. |
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Sigma Aldrich -
C4672
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Application
Cholera toxin B subunit conjugated to peroxidase (CB-HRP) has been shown to be a more effective retrograde and transganglionic tracer for pelvic primary afferents from the urinary bladder than wheat germ agglutinin-horseradish peroxidase or isolectin B4-horseradish peroxidase. Retrograde labeling with CB-HRP has been demonstrated in symphathetic pre-ganglionic neurons as well as in cells of the fastigial nuclei. It is also an effective anterograde tracer for the study of axonal and terminal labeling in brain stem.
Packaging
Vial contains approx. 42 μg cholera toxin B subunit, conjugated to 100 μg (10-30 units) horseradish peroxidase, and buffer salts from 0.1 mL of 0.01 M sodium phosphate, pH 7.5.
Reconstitution
Reconstitute with 0.1 mL water
Unit Definition
One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic. |
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Sigma Aldrich -
C9972
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Analysis Note
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared.
Quality
Biotin content ~1.0 mole/mole protein.
Physical form
Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic. |
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Sigma Aldrich -
C167
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Caution
Do not freeze.
General description
Low salt
Reconstitution
When reconstituted in 0.25 mL distilled water, the compound contains 500 μg of protein in 0.01 M sodium phosphate at pH 7.5.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic. |
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Sigma Aldrich -
C7771
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Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic. |
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Sigma Aldrich -
C1655
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Analysis Note
Activity measured by ELISA using ganglioside GM1 coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic. |