Cholera Toxin B subunit

• Catalog No.: C9903
• M. F.: C16H13ClF3NO2
• M. W.: 343.7281296
• Purity: ≥95% (SDS-PAGE)

SYNONYMS

• IUPAC name: N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxybenzamide
• IUPAC Traditional name: N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxybenzamide
• Synonyms: CTB

DATABASE IDS

• MDL Number: MFCD02356522

PROPERTIES

• Compostion: Protein, ~5% Lowry
• Impurities: ≤0.5% Cholera toxin A subunit (SDS-PAGE)
• Mol. Weight: mol wt ~12 kDa
• Purity: ≥95% (SDS-PAGE)
• Quality Level: PREMIUM
• Apperance: lyophilized powder
• Solubility: H2O: soluble10 mg/mL
• European Hazard Symbols: Harmful (Xn)
• Risk Statements: 20/21/22-36/37/38-52/53
• Safety Statements: 26-36
• Storage Temperature: 2-8°C
• German water hazard class: 2

DETAILS

Description (English)

Analysis Note
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.
Packaging
Package size based on protein content
Reconstitution
When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3 and 1 mM sodium EDTA.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Description (简体中文)

Analysis Note
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.
包装
Package size based on protein content
Reconstitution
When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3 and 1 mM sodium EDTA.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.