7-(dimethylamino)-3H-phenothiazin-3-iminium chloride

• ChemBase ID: 133313
• Molecular Formular: C14H14ClN3S
• Molecular Mass: 291.79906
• Monoisotopic Mass: 291.05969614
• SMILES: CN(C)c1ccc2c(c1)sc1cc(=[NH2+])ccc1n2.[Cl-]
• Canonical SMILES: CN(c1ccc2c(c1)sc1c(n2)ccc(=[NH2+])c1)C.[Cl-]
• InChI: InChI=1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
• InChIKey: NALREUIWICQLPS-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: 7-(dimethylamino)-3H-phenothiazin-3-iminium chloride
• IUPAC Traditional name: 7-(dimethylamino)phenothiazin-3-iminium chloride
• Synonyms: Giemsa stain; Azure mixture sicc. Giemsa stain; Azure eosin methylene blue; Giemsa Stain, Modified Solution; Giemsa stain; Giemsa stain solution; Giemsa Solution; Giemsa stain, modified; 吉氏色素; 吉姆萨染液; Giemsa 染液 溶液; 天青曙红亚甲蓝; Giemsa 染料，改良溶液; Giemsa stain solution

Database IDs

• CAS Number: 51811-82-6
• EC Number: 200-659-6
• MDL Number: MFCD00081642
• PubChem SID: 24895391; 24895379; 24895392; 24847458; 24895390; 24895393; 24847310; 24872445; 162227590
• PubChem CID: 13735

CALCULATED PROPERTIES

• H Acceptors: 2
• H Donor: 1
• LogD (pH = 5.5): -0.7426426
• LogD (pH = 7.4): -0.7249966
• Log P: 2.6730146
• Molar Refractivity: 94.0876 cm^3
• Polarizability: 29.045992 Å^3
• Polar Surface Area: 41.19 Å^2
• Rotatable Bonds: 1
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: ethanol: soluble1 mg/mL
• Apperance: powder
• Melting Point: 300 °C(lit.)
• Flash Point: 11 °C; 48.2 °F; 51.8 °F; 9 °C
• Absorption Wavelength: λmax 521 nm; λmax 648 nm (2nd)

Safety Information

• European Hazard Symbols: Flammable (F); Toxic (T); Harmful (Xn)
• UN Number: 1230
• German water hazard class: 1; 2; 3
• Hazard Class: 3
• Packing Group: 2
• Risk Statements: 11-23/24/25-39/23/24/25; 22-41-52/53; 23/24/25-39/23/24/25
• Safety Statements: 16-36/37-45; 26-39-61; 36/37-45; 7-16-36/37-45
• GHS Pictograms: GHS02; GHS05; GHS06; GHS08
• GHS Signal Word: Danger
• GHS Hazard statements: H225-H301 + H311 + H331-H370; H225-H301-H311-H331-H370; H301-H311-H331-H370; H318
• GHS Precautionary statements: P210-P260-P280-P301 + P310-P311; P260-P280-P301 + P310-P311; P280-P305 + P351 + P338
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves; Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
• RID/ADR: UN 1230 3/PG 2

Product Information

• Concentration: 0.4 % (w/v) in buffered methanol solution, pH 6.8, with stabilizers
• Grade: certified by the Biological Stain Commission; for microscopy (Bact., Hist.); for the staining (of cellular blood components and blood parasites)
• Suitability: passes test for UV spectrum; suitable for cell culture
• Clinical: for in vitro diagnostic use
• Shelf Life: (Expiry date on the label.); (limited shelf life)
• Quality Level: IVD
• Quality: according to Giemsa
• Empirical Formula (Hill Notation): C14H14ClN3S

DETAILS

Sigma Aldrich - GS80

Application
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Sigma Aldrich - GS500

Application
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Sigma Aldrich - 32884

Other Notes
Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.
Principle
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Sigma Aldrich - G5637

包装
5, 25 g in glass bottle
Suitability
Certified for Giemsa stain for blood films and for the Wolbach′s Giemsa method for paraffin sections.

Sigma Aldrich - GS128

Application
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Sigma Aldrich - 12-0440

Suitability
blood stain

Sigma Aldrich - 121398

Packaging
5 g in glass bottle

Sigma Aldrich - 11700

Application
Giemsa stain has been used in a study to develop a fast and easy method for general fluorescent staining of cultured cells on transparent or opaque supports. Giemsa stain has also been used in a study to determine the cell block method using immunostaining for pancreatic juice cytology showed a much lower rate of equivocal borderline malignancy and a tendency for a higher diagnostic yield compared with smear cytology.

Sigma Aldrich - G9641

Application
Used as a chromosome stain and to differentiate nuclear morphology of platelets, RBCs, WBCs, and other cell types.
包装
5, 25 g in glass bottle

Sigma Aldrich - GS1L

Application
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Sigma Aldrich - 48900

Application
Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.
Caution
store at 20°C
Principle
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.
Reconstitution
Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry
Physical form
Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)