Giemsa Stain, Modified Solution

• Catalog No.: 48900
• CAS Number: 51811-82-6
• M. F.: C14H14ClN3S
• M. W.: 291.79906

SYNONYMS

• Title: Giemsa 染料，改良溶液
• IUPAC name: 7-(dimethylamino)-3H-phenothiazin-3-iminium chloride
• IUPAC Traditional name: 7-(dimethylamino)phenothiazin-3-iminium chloride
• Synonyms: Giemsa Solution; Azure eosin methylene blue; Giemsa 染液 溶液; 天青曙红亚甲蓝

DATABASE IDS

• CAS Number: 51811-82-6
• EC Number: 200-659-6
• PubChem SID: 24872445
• MDL Number: MFCD00081642

PROPERTIES

• Grade: for the staining (of cellular blood components and blood parasites)
• Shelf Life: (limited shelf life)
• Flash Point: 9 °C
• Flash Point: 48.2 °F
• GHS Signal Word: Danger
• GHS Hazard statements: H225-H301 + H311 + H331-H370
• European Hazard Symbols: Flammable (F)
• European Hazard Symbols: Toxic (T)
• Personal Protective Equipment: Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
• GHS Precautionary statements: P210-P260-P280-P301 + P310-P311
• RID/ADR: UN 1230 3/PG 2
• Risk Statements: 11-23/24/25-39/23/24/25
• Safety Statements: 7-16-36/37-45
• Hazard Class: 3
• UN Number: 1230
• Packing Group: 2
• German water hazard class: 2

DETAILS

Description (English)

Application
Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.
Caution
store at 20°C
Principle
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.
Reconstitution
Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry
Physical form
Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)

Description (简体中文)

Application
Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.
Caution
store at 20°C
Principle
当 Giemsa 染液对血涂片进行染色时，白细胞的细胞核和细胞质将呈现特征性的蓝色或粉红色。使用经纯化的伊红和噻嗪染料可使批间差异最小化。
Reconstitution
Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry
Physical form
Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)