2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2$l^{5},3,4-tetrazol-2-ylium bromide

• ChemBase ID: 102580
• Molecular Formular: C18H16BrN5S
• Molecular Mass: 414.32214
• Monoisotopic Mass: 413.03097854
• SMILES: [Br-].Cc1c(C)nc(s1)[n+]1nc(nn1c1ccccc1)c1ccccc1
• Canonical SMILES: Cc1sc(nc1C)[n+]1nc(nn1c1ccccc1)c1ccccc1.[Br-]
• InChI: InChI=1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
• InChIKey: AZKSAVLVSZKNRD-UHFFFAOYSA-M

NAMES AND DATABASE IDS

Names

• IUPAC name: 2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2$l^{5},3,4-tetrazol-2-ylium bromide; 2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2λ^5,3,4-tetrazol-2-ylium bromide
• IUPAC Traditional name: 2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-1,2$l^{5},3,4-tetrazol-2-ylium bromide; 2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-1,2λ^5,3,4-tetrazol-2-ylium bromide
• Synonyms: MTT; Thiazolyl Blue Tetrazolium Bromide; Methylthiazolyldiphenyl-tetrazolium bromide; 3-(4,5-DIMETHYLTHIAZOLYL-2)-2,5-DIPHENYL TETRAZOLIUM BROMIDE; Thiazolyl Blue; 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; Thiazolyl Blue Tetrazolium Bromide; 噻唑蓝溴化四唑

Database IDs

• CAS Number: 298-93-1
• EC Number: 206-069-5
• MDL Number: MFCD00011964
• Beilstein Number: 3825277; 4081397
• PubChem SID: 24897030; 24889092; 24896603; 162090196; 24848194
• PubChem CID: 64965

CALCULATED PROPERTIES

• H Acceptors: 3
• H Donor: 0
• LogD (pH = 5.5): 2.741026
• LogD (pH = 7.4): 2.741026
• Log P: 2.741026
• Molar Refractivity: 128.6325 cm^3
• Polarizability: 37.12812 Å^3
• Polar Surface Area: 47.48 Å^2
• Rotatable Bonds: 3
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: H2O: soluble5 mg/mL; methanol: soluble5 mg/mL, clear
• Apperance: powder
• Melting Point: 195 °C (dec.)(lit.); 195°C; ca 195°C dec.
• Absorption Wavelength: λmax 378 nm

Safety Information

• Storage Condition: 2-8°C, Protect from light
• Storage Warning: Light Sensitive
• RTECS: XF8060000
• European Hazard Symbols: Irritant (Xi); Harmful (Xn)
• German water hazard class: 3
• Risk Statements: 36/37/38; 36/37/38-68
• Safety Statements: 26; 26-36/37; 26-37-60
• TSCA Listed: 是
• GHS Pictograms: GHS07; GHS08
• GHS Signal Word: Warning
• GHS Hazard statements: H315-H319-H335-H341
• GHS Precautionary statements: P261-P281-P305 + P351 + P338
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: 2-8°C

Product Information

• Purity: ~98%; ≥97.0% (enzymatic); ≥97.5% (HPLC); 98%
• Suitability: suitable for cell culture; suitable for insect cell culture
• Product Line: BioReagent
• Empirical Formula (Hill Notation): C18H16BrN5S

DETAILS

MP Biomedicals - 02194592

Cell Culture Reagent
Yellow crystals
Purity: ~98%

MP Biomedicals - 02102227

Yellow crystals
Purity: ~98%

Sigma Aldrich - M5655

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Use as a colorimetric metabolic activity indicator in cell viability assays.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9
Packaging
1 g in glass bottle
15, 100, 500 mg in glass bottle

Sigma Aldrich - M2128

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Packaging
1, 5, 10 g in glass bottle
100, 250, 500 mg in glass bottle
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9

Sigma Aldrich - 135038

Packaging
1 g in glass bottle
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9

Sigma Aldrich - 88415

Other Notes
Review: Tetrazolium salts and formazans10
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9