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Thiazolyl Blue Tetrazolium Bromide_Molecular_structure_CAS_298-93-1)
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Thiazolyl Blue Tetrazolium Bromide

Catalog No. M5655 Name Sigma Aldrich
CAS Number 298-93-1 Website http://www.sigmaaldrich.com
M. F. C18H16BrN5S Telephone 1-800-521-8956
M. W. 414.32214 Fax
Purity ≥97.5% (HPLC) Email
Storage Chembase ID: 102580

SYNONYMS

IUPAC name
2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2λ5,3,4-tetrazol-2-ylium bromide
IUPAC Traditional name
2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-1,2λ5,3,4-tetrazol-2-ylium bromide
Synonyms
MTT
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
Methylthiazolyldiphenyl-tetrazolium bromide

DATABASE IDS

EC Number 206-069-5
CAS Number 298-93-1
MDL Number MFCD00011964
PubChem SID 24897030
Beilstein Number 4081397

PROPERTIES

Empirical Formula (Hill Notation) C18H16BrN5S
Product Line BioReagent
Purity ≥97.5% (HPLC)
Suitability suitable for cell culture
Suitability suitable for insect cell culture
Apperance powder
Melting Point 195 °C (dec.)(lit.)
Solubility H2O: soluble5 mg/mL
GHS Pictograms GHS07
GHS Pictograms GHS08
GHS Signal Word Warning
GHS Hazard statements H315-H319-H335-H341
European Hazard Symbols Harmful Harmful (Xn)
MSDS Link Download
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
GHS Precautionary statements P261-P281-P305 + P351 + P338
Risk Statements 36/37/38-68
RTECS XF8060000
Safety Statements 26-36/37
Storage Temperature 2-8°C
German water hazard class 3

DETAILS

Description (English)
Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Use as a colorimetric metabolic activity indicator in cell viability assays.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9
Packaging
1 g in glass bottle
15, 100, 500 mg in glass bottle
Description (简体中文)
Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Use as a colorimetric metabolic activity indicator in cell viability assays.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9
包装
1 g in glass bottle
15, 100, 500 mg in glass bottle

REFERENCES