Thiazolyl Blue Tetrazolium Bromide

• Catalog No.: 88415
• CAS Number: 298-93-1
• M. F.: C18H16BrN5S
• M. W.: 414.32214
• Purity: ≥97.0% (enzymatic)

SYNONYMS

• IUPAC name: 2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2λ^5,3,4-tetrazol-2-ylium bromide
• IUPAC Traditional name: 2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-1,2λ^5,3,4-tetrazol-2-ylium bromide
• Synonyms: MTT; 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide

DATABASE IDS

• PubChem SID: 24889092
• EC Number: 206-069-5
• CAS Number: 298-93-1
• MDL Number: MFCD00011964
• Beilstein Number: 4081397

PROPERTIES

• Empirical Formula (Hill Notation): C18H16BrN5S
• Purity: ≥97.0% (enzymatic)
• Melting Point: 195 °C (dec.)(lit.)
• Solubility: methanol: soluble5 mg/mL, clear
• GHS Signal Word: Warning
• GHS Hazard statements: H315-H319-H335-H341
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• GHS Precautionary statements: P261-P281-P305 + P351 + P338
• Risk Statements: 36/37/38
• RTECS: XF8060000
• Safety Statements: 26
• German water hazard class: 3

DETAILS

Description (English)

Other Notes
Review: Tetrazolium salts and formazans10
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9

Description (简体中文)

Other Notes
Review: Tetrazolium salts and formazans10
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9