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1064-48-8 molecular structure
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disodium 4-amino-5-hydroxy-3-[2-(4-nitrophenyl)diazen-1-yl]-6-(2-phenyldiazen-1-yl)naphthalene-2,7-disulfonate

ChemBase ID: 102209
Molecular Formular: C22H14N6Na2O9S2
Molecular Mass: 616.4909
Monoisotopic Mass: 616.00590662
SMILES and InChIs

SMILES:
[Na+].[Na+].Nc1c2c(O)c(/N=N/c3ccccc3)c(cc2cc(c1/N=N/c1ccc(cc1)[N+](=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-]
Canonical SMILES:
[O-][N+](=O)c1ccc(cc1)/N=N/c1c(N)c2c(cc1S(=O)(=O)[O-])cc(c(c2O)/N=N/c1ccccc1)S(=O)(=O)[O-].[Na+].[Na+]
InChI:
InChI=1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2
InChIKey:
AOMZHDJXSYHPKS-UHFFFAOYSA-L

Cite this record

CBID:102209 http://www.chembase.cn/molecule-102209.html

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NAMES AND DATABASE IDS

NAMES AND DATABASE IDS

Names Database IDs
IUPAC name
disodium 4-amino-5-hydroxy-3-[2-(4-nitrophenyl)diazen-1-yl]-6-(2-phenyldiazen-1-yl)naphthalene-2,7-disulfonate
disodium 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazen-1-yl]-6-[(E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate
IUPAC Traditional name
disodium 4-amino-5-hydroxy-3-[2-(4-nitrophenyl)diazen-1-yl]-6-(2-phenyldiazen-1-yl)naphthalene-2,7-disulfonate
dipotassium 4-amino-5-hydroxy-3-[2-(4-nitrophenyl)diazen-1-yl]-6-(2-phenyldiazen-1-yl)naphthalene-2,7-disulfonate
disodium 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazen-1-yl]-6-[(E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate
Synonyms
Naphthalene Black 10B
Amido Black 10B
NAPHTHOL BLUE BLACK
Amidoschwarz
Acidal Black 10B
Acidal Navy Blue 3BR
Naphthalene Black 10B (see talk page)
Naphthol Blue Black
Buffalo Black NBR
C.I. 20470
Acid Black 1
AMIDO BLACK 10B
Amido Black 10B
Naphthol Blue Black
Naphthol Blue Black solution
Amido Black Staining Solution 2X
萘黑10B
CAS Number
1064-48-8
EC Number
213-903-1
MDL Number
MFCD00004017
Beilstein Number
5374263
PubChem SID
162089582
24851681
24891210
24897642
24897883
PubChem CID
9566044
5359556
Chemspider ID
10660833
Wikipedia Title
Amido_black_10B
Color Index Number
20470

CALCULATED PROPERTIES

CALCULATED PROPERTIES

JChem
Acid pKa -3.1733475  H Acceptors 14 
H Donor LogD (pH = 5.5) 0.19101588 
LogD (pH = 7.4) 0.19059597  Log P 0.776995 
Molar Refractivity 144.1538 cm3 Polarizability 53.10609 Å3
Polar Surface Area 255.91 Å2 Rotatable Bonds
Lipinski's Rule of Five false 

PROPERTIES

PROPERTIES

Physical Property Safety Information Product Information Bioassay(PubChem)
Solubility
~ 30 g/l at 20 °C in water expand Show data source
Apperance
Dark red to black expand Show data source
Flash Point
30 °C expand Show data source
86 °F expand Show data source
Absorption Wavelength
λmax 618 nm expand Show data source
Storage Condition
Room Temperature (15-30°C) expand Show data source
Storage Warning
Hygroscopic expand Show data source
RTECS
QJ6196000 expand Show data source
European Hazard Symbols
Irritant Irritant (Xi) expand Show data source
UN Number
2924 expand Show data source
MSDS Link
Download expand Show data source
Download expand Show data source
Download expand Show data source
Download expand Show data source
Download expand Show data source
Download expand Show data source
German water hazard class
2 expand Show data source
3 expand Show data source
Hazard Class
3 expand Show data source
Packing Group
3 expand Show data source
Risk Statements
10-36/38-67 expand Show data source
36/37/38 expand Show data source
R:36/37/38 expand Show data source
R20, R21, R33 expand Show data source
Safety Statements
26 expand Show data source
26-37 expand Show data source
S:20-25-26-37/39 expand Show data source
S28A, S37, S45 expand Show data source
TSCA Listed
expand Show data source
GHS Pictograms
GHS02 expand Show data source
GHS07 expand Show data source
GHS Signal Word
Warning expand Show data source
NFPA704
NFPA 704 diagram
0
2
0
expand Show data source
GHS Hazard statements
H226-H315-H319-H336 expand Show data source
H315-H319-H335 expand Show data source
GHS Precautionary statements
P261-P305 + P351 + P338 expand Show data source
P261-P305+P351+P338-P302+P352-P321-P405-P501A expand Show data source
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves expand Show data source
RID/ADR
UN 2924 3/PG 3 expand Show data source
Purity
~50% (dye content) expand Show data source
Dye Content ~85% expand Show data source
Grade
for microscopy (Hist.) expand Show data source
Compostion
Dye content, ~50% expand Show data source
Dye content, ~85% expand Show data source
Dye content, 80% expand Show data source
Certificate of Analysis
Download expand Show data source
Download expand Show data source
Download expand Show data source
Suitability
suitable for electrophoresis expand Show data source
Impurities
≤10% water expand Show data source
Empirical Formula (Hill Notation)
C22H14N6Na2O9S2 expand Show data source

DETAILS

DETAILS

MP Biomedicals MP Biomedicals Wikipedia Wikipedia Sigma Aldrich Sigma Aldrich
MP Biomedicals - 02100563 external link
Useful dye for staining protein patterns after electrophoresis. Stains proteins, histones, orosomucoids. Also used on SDS-gels. Dye content: ~ 85%
MP Biomedicals - 04806717 external link
General protein and nucleic acid stain for SDS-PAGE gels. Useful in staining not only proteins, but also histones and orosomucoid.
MP Biomedicals - 04806712 external link
General protein and nucleic acid stain for SDS-PAGE gels. Useful in staining not only proteins, but also histones and orosomucoid.
Sigma Aldrich - A8181 external link
Application
Naphthol Blue Black stain is designed for rapid staining of protein bands on nitrocellulose membranes (Western blots).1 Blots are stained for 1 min and then destained for 30 minutes in 25% (v/v) isopropanol, 10% (v/v) acetic acid. Used to detect proteins in the lower microgram range with a clear background. Naphthol Blue Black can also be used for staining PAGE gels.2
Reconstitution
After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.
Sigma Aldrich - N3393 external link
Application
Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.
包装
25, 100 g in glass bottle
Sigma Aldrich - 195243 external link
Packaging
25, 100 g in glass bottle

REFERENCES

REFERENCES

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PATENTS

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