disodium 4-amino-5-hydroxy-3-[2-(4-nitrophenyl)diazen-1-yl]-6-(2-phenyldiazen-1-yl)naphthalene-2,7-disulfonate

• ChemBase ID: 102209
• Molecular Formular: C22H14N6Na2O9S2
• Molecular Mass: 616.4909
• Monoisotopic Mass: 616.00590662
• SMILES: [Na+].[Na+].Nc1c2c(O)c(/N=N/c3ccccc3)c(cc2cc(c1/N=N/c1ccc(cc1)[N+](=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-]
• Canonical SMILES: [O-][N+](=O)c1ccc(cc1)/N=N/c1c(N)c2c(cc1S(=O)(=O)[O-])cc(c(c2O)/N=N/c1ccccc1)S(=O)(=O)[O-].[Na+].[Na+]
• InChI: InChI=1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2
• InChIKey: AOMZHDJXSYHPKS-UHFFFAOYSA-L

NAMES AND DATABASE IDS

Names

• IUPAC name: disodium 4-amino-5-hydroxy-3-[2-(4-nitrophenyl)diazen-1-yl]-6-(2-phenyldiazen-1-yl)naphthalene-2,7-disulfonate; disodium 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazen-1-yl]-6-[(E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate
• IUPAC Traditional name: disodium 4-amino-5-hydroxy-3-[2-(4-nitrophenyl)diazen-1-yl]-6-(2-phenyldiazen-1-yl)naphthalene-2,7-disulfonate; dipotassium 4-amino-5-hydroxy-3-[2-(4-nitrophenyl)diazen-1-yl]-6-(2-phenyldiazen-1-yl)naphthalene-2,7-disulfonate; disodium 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazen-1-yl]-6-[(E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate
• Synonyms: Naphthalene Black 10B; Amido Black 10B; NAPHTHOL BLUE BLACK; Amidoschwarz; Acidal Black 10B; Acidal Navy Blue 3BR; Naphthalene Black 10B (see talk page); Naphthol Blue Black; Buffalo Black NBR; C.I. 20470; Acid Black 1; AMIDO BLACK 10B; Amido Black 10B; Naphthol Blue Black; Naphthol Blue Black solution; Amido Black Staining Solution 2X; 萘黑10B

Database IDs

• CAS Number: 1064-48-8
• EC Number: 213-903-1
• MDL Number: MFCD00004017
• Beilstein Number: 5374263
• PubChem SID: 24897883; 162089582; 24851681; 24891210; 24897642
• PubChem CID: 9566044; 5359556
• Chemspider ID: 10660833
• Wikipedia Title: Amido_black_10B
• Color Index Number: 20470

CALCULATED PROPERTIES

• Acid pKa: -3.1733475
• H Acceptors: 14
• H Donor: 2
• LogD (pH = 5.5): 0.19101588
• LogD (pH = 7.4): 0.19059597
• Log P: 0.776995
• Molar Refractivity: 144.1538 cm^3
• Polarizability: 53.10609 Å^3
• Polar Surface Area: 255.91 Å^2
• Rotatable Bonds: 7
• Lipinski's Rule of Five: false

PROPERTIES

Physical Property

• Solubility: ~ 30 g/l at 20 °C in water
• Apperance: Dark red to black
• Flash Point: 30 °C; 86 °F
• Absorption Wavelength: λmax 618 nm

Safety Information

• Storage Condition: Room Temperature (15-30°C)
• Storage Warning: Hygroscopic
• RTECS: QJ6196000
• European Hazard Symbols: Irritant (Xi)
• UN Number: 2924
• German water hazard class: 2; 3
• Hazard Class: 3
• Packing Group: 3
• Risk Statements: 10-36/38-67; 36/37/38; R:36/37/38; R20, R21, R33
• Safety Statements: 26; 26-37; S:20-25-26-37/39; S28A, S37, S45
• TSCA Listed: 是
• GHS Pictograms: GHS02; GHS07
• GHS Signal Word: Warning
• NFPA704:
  

  0

  2

  0
• GHS Hazard statements: H226-H315-H319-H336; H315-H319-H335
• GHS Precautionary statements: P261-P305 + P351 + P338; P261-P305+P351+P338-P302+P352-P321-P405-P501A
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• RID/ADR: UN 2924 3/PG 3

Product Information

• Purity: ~50% (dye content); Dye Content ~85%
• Grade: for microscopy (Hist.)
• Compostion: Dye content, ~50%; Dye content, ~85%; Dye content, 80%
• Suitability: suitable for electrophoresis
• Impurities: ≤10% water
• Empirical Formula (Hill Notation): C22H14N6Na2O9S2

DETAILS

MP Biomedicals - 02100563

Useful dye for staining protein patterns after electrophoresis. Stains proteins, histones, orosomucoids. Also used on SDS-gels. Dye content: ~ 85%

MP Biomedicals - 04806717

General protein and nucleic acid stain for SDS-PAGE gels. Useful in staining not only proteins, but also histones and orosomucoid.

MP Biomedicals - 04806712

General protein and nucleic acid stain for SDS-PAGE gels. Useful in staining not only proteins, but also histones and orosomucoid.

Sigma Aldrich - A8181

Application
Naphthol Blue Black stain is designed for rapid staining of protein bands on nitrocellulose membranes (Western blots).1 Blots are stained for 1 min and then destained for 30 minutes in 25% (v/v) isopropanol, 10% (v/v) acetic acid. Used to detect proteins in the lower microgram range with a clear background. Naphthol Blue Black can also be used for staining PAGE gels.2
Reconstitution
After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.

Sigma Aldrich - N3393

Application
Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.
包装
25, 100 g in glass bottle

Sigma Aldrich - 195243

Packaging
25, 100 g in glass bottle