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Naphthol Blue Black

Catalog No. N3393 Name Sigma Aldrich
CAS Number 1064-48-8 Website http://www.sigmaaldrich.com
M. F. C22H14N6Na2O9S2 Telephone 1-800-521-8956
M. W. 616.4909 Fax
Purity Email
Storage Chembase ID: 102209


IUPAC name
disodium 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazen-1-yl]-6-[(E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate
IUPAC Traditional name
disodium 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazen-1-yl]-6-[(E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate
Amido Black 10B
Acid Black 1
Buffalo Black NBR


CAS Number 1064-48-8
MDL Number MFCD00004017
EC Number 213-903-1
PubChem SID 24897642
Color Index Number 20470
Beilstein Number 5374263


Compostion Dye content, ~85%
Impurities ≤10% water
Suitability suitable for electrophoresis
GHS Pictograms GHS07
GHS Signal Word Warning
GHS Hazard statements H315-H319-H335
European Hazard Symbols Irritant Irritant (Xi)
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves
GHS Precautionary statements P261-P305 + P351 + P338
Risk Statements 36/37/38
RTECS QJ6196000
Safety Statements 26
German water hazard class 3


Description (简体中文)
Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.
25, 100 g in glass bottle