Amido Black Staining Solution 2X

• Catalog No.: A8181
• CAS Number: 1064-48-8
• M. F.: C22H14N6Na2O9S2
• M. W.: 616.4909

SYNONYMS

• IUPAC name: disodium 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazen-1-yl]-6-[(E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate
• IUPAC Traditional name: disodium 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazen-1-yl]-6-[(E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate
• Synonyms: Amido Black 10B; Naphthol Blue Black solution

DATABASE IDS

• CAS Number: 1064-48-8
• MDL Number: MFCD00004017
• PubChem SID: 24891210
• Beilstein Number: 5374263

PROPERTIES

• Flash Point: 30 °C
• Flash Point: 86 °F
• GHS Signal Word: Warning
• GHS Hazard statements: H226-H315-H319-H336
• European Hazard Symbols: Irritant (Xi)
• GHS Precautionary statements: P261-P305 + P351 + P338
• RID/ADR: UN 2924 3/PG 3
• Risk Statements: 10-36/38-67
• Safety Statements: 26
• Hazard Class: 3
• UN Number: 2924
• Packing Group: 3
• German water hazard class: 2

DETAILS

Description (English)

Application
Naphthol Blue Black stain is designed for rapid staining of protein bands on nitrocellulose membranes (Western blots).1 Blots are stained for 1 min and then destained for 30 minutes in 25% (v/v) isopropanol, 10% (v/v) acetic acid. Used to detect proteins in the lower microgram range with a clear background. Naphthol Blue Black can also be used for staining PAGE gels.2
Reconstitution
After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.