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Amido Black Staining Solution 2X_Molecular_structure_CAS_1064-48-8)
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Amido Black Staining Solution 2X

Catalog No. A8181 Name Sigma Aldrich
CAS Number 1064-48-8 Website http://www.sigmaaldrich.com
M. F. C22H14N6Na2O9S2 Telephone 1-800-521-8956
M. W. 616.4909 Fax
Purity Email
Storage Chembase ID: 102209


IUPAC name
disodium 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazen-1-yl]-6-[(E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate
IUPAC Traditional name
disodium 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazen-1-yl]-6-[(E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate
Amido Black 10B
Naphthol Blue Black solution


CAS Number 1064-48-8
MDL Number MFCD00004017
PubChem SID 24891210
Beilstein Number 5374263


Flash Point 30 °C
Flash Point 86 °F
GHS Pictograms GHS02
GHS Pictograms GHS07
GHS Signal Word Warning
GHS Hazard statements H226-H315-H319-H336
European Hazard Symbols Irritant Irritant (Xi)
GHS Precautionary statements P261-P305 + P351 + P338
RID/ADR UN 2924 3/PG 3
Risk Statements 10-36/38-67
Safety Statements 26
Hazard Class 3
UN Number 2924
Packing Group 3
German water hazard class 2


Description (English)
Naphthol Blue Black stain is designed for rapid staining of protein bands on nitrocellulose membranes (Western blots).1 Blots are stained for 1 min and then destained for 30 minutes in 25% (v/v) isopropanol, 10% (v/v) acetic acid. Used to detect proteins in the lower microgram range with a clear background. Naphthol Blue Black can also be used for staining PAGE gels.2
After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.