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Urea

Catalog No. 15604 Name Sigma Aldrich
CAS Number 57-13-6 Website http://www.sigmaaldrich.com
M. F. CH4N2O Telephone 1-800-521-8956
M. W. 60.05526 Fax
Purity 99.0-100.5% Email
Storage Chembase ID: 3541

SYNONYMS

Title
尿素
IUPAC name
urea
IUPAC Traditional name
urea
Synonyms
Carbamide
碳酰胺
碳酰二胺
Carbonyldiamide

DATABASE IDS

EC Number 200-315-5
MDL Number MFCD00008022
Beilstein Number 635724
CAS Number 57-13-6

PROPERTIES

Impurities ≤0.001% heavy metals (as Pb)
Impurities ≤0.04% insoluble in ethanol
Impurities ≤0.05% ammonium (NH4)
Impurities ≤0.1% biuret(carbamylurea)
Linear Formula NH2CONH2
Loss on Drying ≤1.0% loss on drying, 105 °C, 1 h
Purity 99.0-100.5%
Purity 99.0-101.0% (calc. on dry substance)
Quality meets analytical specification of Ph. Eur., BP, USP
Suitability in accordance for appearance of solution
Suitability in accordance for identity
Antion Traces chloride (Cl-): ≤10 mg/kg
Antion Traces sulfate (SO42-): ≤50 mg/kg
Cation Traces Fe: ≤0.5 mg/kg
Grade puriss.
Ignition Residue ≤0.1% (as SO4)
Impurities alkalic reac. substances, complies
Impurities residual solvents, complies
Density 1.335 g/mL at 25 °C(lit.)
Melting Point 132-135 °C(lit.)
Melting Point 132-135 °C
MSDS Link Download
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RTECS YR6250000
German water hazard class 1

DETAILS

Description (English)
Application
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
Description (简体中文)
Application
用于蛋白质变性以及作为不溶性或变性蛋白的温和增溶剂。可用于从已由 6M 盐酸胍变性的样品(如包涵体)复性蛋白质。可与盐酸胍和二硫苏糖醇 (DTT) 一起用于复性变性蛋白以恢复其天然或活性形式。

REFERENCES