o-Phenylenediamine dihydrochloride

• Catalog No.: P4664
• CAS Number: 615-28-1
• M. F.: C6H10Cl2N2
• M. W.: 181.063

SYNONYMS

• Title: 邻苯二胺 二盐酸盐
• IUPAC name: benzene-1,2-diamine dihydrochloride
• IUPAC Traditional name: o-phenylenediamine dihydrochloride

DATABASE IDS

• PubChem SID: 24898491
• CAS Number: 615-28-1
• Beilstein Number: 3912045
• MDL Number: MFCD00012966

PROPERTIES

• Linear Formula: C6H4(NH2)2·2HCl
• Apperance: tablet
• Melting Point: 258 °C (dec.)(lit.)
• GHS Signal Word: Warning
• GHS Hazard statements: H302-H317-H319-H332-H341-H351-H410
• European Hazard Symbols: Toxic (T)
• European Hazard Symbols: Nature polluting (N)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
• GHS Precautionary statements: P273-P280-P305 + P351 + P338-P501
• Risk Statements: 20/21-25-36-40-43-50/53-68
• Safety Statements: 26-36/37-45-61
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Description (简体中文)

Application
邻苯二胺是一种适用于 ELISA 分析的过氧化物酶底物。它可生成黄褐色的可溶性终产物，而该产物可通过分光光度法在 450nm 处读取。可用 3N HCl 或 3M H2SO4 中止 OPD 反应，并在 492nm 处读取。
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.