benzene-1,2-diamine dihydrochloride

• ChemBase ID: 105252
• Molecular Formular: C6H10Cl2N2
• Molecular Mass: 181.063
• Monoisotopic Mass: 180.02210369
• SMILES: Cl.Cl.Nc1c(N)cccc1
• Canonical SMILES: Nc1ccccc1N.Cl.Cl
• InChI: InChI=1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
• InChIKey: RIIWUGSYXOBDMC-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: benzene-1,2-diamine dihydrochloride
• IUPAC Traditional name: o-phenylenediamine dihydrochloride
• Synonyms: o-Phenylenediamine dihydrochloride; OPD Tablets; o-PHENYLENEDIAMINE DIHYDROCHLORIDE TABLETS; o-PHENYLENEDIAMINE DIHYDROCHLORIDE; 邻苯二胺 二盐酸盐

Database IDs

• CAS Number: 615-28-1
• EC Number: 210-418-7
• MDL Number: MFCD00012966
• Beilstein Number: 3912045
• PubChem SID: 24887303; 24887307; 24898491; 24898919; 24898981; 24898837; 24887302; 24898741; 24898787; 24898149; 162092071; 24898805; 24898457; 24898213; 24898798; 24898979
• PubChem CID: 11990

CALCULATED PROPERTIES

• H Acceptors: 2
• H Donor: 2
• LogD (pH = 5.5): 0.24660818
• LogD (pH = 7.4): 0.31445572
• Log P: 0.3153939
• Molar Refractivity: 35.4588 cm^3
• Polarizability: 12.615475 Å^3
• Polar Surface Area: 52.04 Å^2
• Rotatable Bonds: 0
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: H2O: soluble1 tablet/10 mL, clear, colorless
• Apperance: tablet; white to off-white
• Melting Point: 258 °C (dec.)(lit.); 258°C (dec.)

Safety Information

• Storage Condition: 0°C, Warm to room temperature before opening; 2-8°C, Store Under Nitrogen, Protect from light
• RTECS: ST0175000
• European Hazard Symbols: Nature polluting (N); Toxic (T); Harmful (Xn)
• UN Number: 1673; 2811
• German water hazard class: 3
• Hazard Class: 6.1
• Packing Group: 3; III
• Australian Hazchem: 2X
• Risk Statements: 20/21-25-36-40-43-50/53-68; 22-36-40-43-51/53-68; 22-40-43-51/53-68; R:20/21-25-36-40-43-50/53
• Safety Statements: 26-36/37-45-61; 26-36/37-61; 28-36/37-45-60-61; 36/37-61; S:28-45-60-61-36/37
• EU Classification: T2
• EU Hazard Identification Number: 6.1B
• Emergency Response Guidebook(ERG) Number: 153
• GHS Pictograms: GHS06; GHS07; GHS08; GHS09
• GHS Signal Word: Danger; Warning
• GHS Hazard statements: H301-H312-H317-H319-H332-H341-H351-H410; H302-H317-H319-H332-H341-H351-H410; H317-H319-H341-H351-H411; H317-H341-H351-H411
• GHS Precautionary statements: P273-P280; P273-P280-P301 + P310-P305 + P351 + P338-P501; P273-P280-P305 + P351 + P338; P273-P280-P305 + P351 + P338-P501
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
• RID/ADR: UN 1673 6.1/PG 3; UN 2811 6.1/PG 3
• Storage Temperature: -20°C; 2-8°C

Product Information

• Purity: ≥99%; ≥99.0% (AT); 99%
• Grade: for fluorescence
• Quality Level: PREMIUM
• Linear Formula: C6H4(NH2)2·2HCl

DETAILS

MP Biomedicals - 02195379

Dihydrochloride
Purity: 99+%

MP Biomedicals - 02159520

(OPD Tablets) Peroxidase substrate for EIA procedures. Concentration: >13 mg/tablet. Unit Definition: One tablet will stabilize 50 ml of 0.1M Phosphate Buffer Solution. Clear solubility in water with pH 3.7-4.6 at 25°C.

MP Biomedicals - 02198808

(OPD Tablets)
Peroxidase substrate for EIA procedures.
Concentration: 10 mg/tablet.

Sigma Aldrich - P8287

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Sigma Aldrich - P1526

Application
邻苯二胺是一种适用于 ELISA 分析的过氧化物酶底物。它可生成黄褐色的可溶性终产物，而该产物可通过分光光度法在 450nm 处读取。可用 3N HCl 或 3M H2SO4 中止 OPD 反应，并在 492nm 处读取。
Caution
储存时颜色可能变深。

Sigma Aldrich - P8806

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Sigma Aldrich - P6787

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Sigma Aldrich - P8412

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Sigma Aldrich - P6912

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Packaging
150 mg total tablet weight
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Sigma Aldrich - 78441

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Other Notes
Gas-liquid chromatography of α-ketoacids as their O-trimethylsilyl-quinoxalinol derivatives1

Sigma Aldrich - 78440

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Caution
may discolor to violet on storage
Packaging
50 g in glass bottle

Sigma Aldrich - P3804

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Packaging
16 mg total tablet weight
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Sigma Aldrich - 78449

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

Sigma Aldrich - 238236

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

Sigma Aldrich - P4664

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Sigma Aldrich - P6662

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Sigma Aldrich - P7288

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Sigma Aldrich - P8787

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Sigma Aldrich - P1063

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.