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615-28-1 molecular structure
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benzene-1,2-diamine dihydrochloride

ChemBase ID: 105252
Molecular Formular: C6H10Cl2N2
Molecular Mass: 181.063
Monoisotopic Mass: 180.02210369
SMILES and InChIs

SMILES:
Cl.Cl.Nc1c(N)cccc1
Canonical SMILES:
Nc1ccccc1N.Cl.Cl
InChI:
InChI=1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChIKey:
RIIWUGSYXOBDMC-UHFFFAOYSA-N

Cite this record

CBID:105252 http://www.chembase.cn/molecule-105252.html

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NAMES AND DATABASE IDS

NAMES AND DATABASE IDS

Names Database IDs
IUPAC name
benzene-1,2-diamine dihydrochloride
IUPAC Traditional name
o-phenylenediamine dihydrochloride
Synonyms
o-Phenylenediamine dihydrochloride
OPD Tablets
o-PHENYLENEDIAMINE DIHYDROCHLORIDE TABLETS
o-PHENYLENEDIAMINE DIHYDROCHLORIDE
邻苯二胺 二盐酸盐
CAS Number
615-28-1
EC Number
210-418-7
MDL Number
MFCD00012966
Beilstein Number
3912045
PubChem SID
24887303
24898837
24887302
24887307
24898491
24898919
24898981
24898741
24898787
24898149
162092071
24898805
24898457
24898213
24898798
24898979
PubChem CID
11990

CALCULATED PROPERTIES

CALCULATED PROPERTIES

JChem
H Acceptors H Donor
LogD (pH = 5.5) 0.24660818  LogD (pH = 7.4) 0.31445572 
Log P 0.3153939  Molar Refractivity 35.4588 cm3
Polarizability 12.615475 Å3 Polar Surface Area 52.04 Å2
Rotatable Bonds Lipinski's Rule of Five true 

PROPERTIES

PROPERTIES

Physical Property Safety Information Product Information Bioassay(PubChem)
Solubility
H2O: soluble1 tablet/10 mL, clear, colorless expand Show data source
Apperance
tablet expand Show data source
white to off-white expand Show data source
Melting Point
258 °C (dec.)(lit.) expand Show data source
258°C (dec.) expand Show data source
Storage Condition
0°C, Warm to room temperature before opening expand Show data source
2-8°C, Store Under Nitrogen, Protect from light expand Show data source
RTECS
ST0175000 expand Show data source
European Hazard Symbols
Nature polluting Nature polluting (N) expand Show data source
Toxic Toxic (T) expand Show data source
Harmful Harmful (Xn) expand Show data source
UN Number
1673 expand Show data source
2811 expand Show data source
MSDS Link
Download expand Show data source
Download expand Show data source
Download expand Show data source
Download expand Show data source
Download expand Show data source
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Download expand Show data source
Download expand Show data source
Download expand Show data source
Download expand Show data source
Download expand Show data source
Download expand Show data source
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Download expand Show data source
Download expand Show data source
Download expand Show data source
Download expand Show data source
German water hazard class
3 expand Show data source
Hazard Class
6.1 expand Show data source
Packing Group
3 expand Show data source
III expand Show data source
Australian Hazchem
2X expand Show data source
Risk Statements
20/21-25-36-40-43-50/53-68 expand Show data source
22-36-40-43-51/53-68 expand Show data source
22-40-43-51/53-68 expand Show data source
R:20/21-25-36-40-43-50/53 expand Show data source
Safety Statements
26-36/37-45-61 expand Show data source
26-36/37-61 expand Show data source
28-36/37-45-60-61 expand Show data source
36/37-61 expand Show data source
S:28-45-60-61-36/37 expand Show data source
EU Classification
T2 expand Show data source
EU Hazard Identification Number
6.1B expand Show data source
Emergency Response Guidebook(ERG) Number
153 expand Show data source
GHS Pictograms
GHS06 expand Show data source
GHS07 expand Show data source
GHS08 expand Show data source
GHS09 expand Show data source
GHS Signal Word
Danger expand Show data source
Warning expand Show data source
GHS Hazard statements
H301-H312-H317-H319-H332-H341-H351-H410 expand Show data source
H302-H317-H319-H332-H341-H351-H410 expand Show data source
H317-H319-H341-H351-H411 expand Show data source
H317-H341-H351-H411 expand Show data source
GHS Precautionary statements
P273-P280 expand Show data source
P273-P280-P301 + P310-P305 + P351 + P338-P501 expand Show data source
P273-P280-P305 + P351 + P338 expand Show data source
P273-P280-P305 + P351 + P338-P501 expand Show data source
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges expand Show data source
RID/ADR
UN 1673 6.1/PG 3 expand Show data source
UN 2811 6.1/PG 3 expand Show data source
Storage Temperature
-20°C expand Show data source
2-8°C expand Show data source
Purity
≥99% expand Show data source
≥99.0% (AT) expand Show data source
99% expand Show data source
Grade
for fluorescence expand Show data source
Certificate of Analysis
Download expand Show data source
Download expand Show data source
Download expand Show data source
Quality Level
PREMIUM expand Show data source
Linear Formula
C6H4(NH2)2·2HCl expand Show data source

DETAILS

DETAILS

MP Biomedicals MP Biomedicals Sigma Aldrich Sigma Aldrich
MP Biomedicals - 02195379 external link
Dihydrochloride
Purity: 99+%
MP Biomedicals - 02159520 external link
(OPD Tablets) Peroxidase substrate for EIA procedures. Concentration: >13 mg/tablet. Unit Definition: One tablet will stabilize 50 ml of 0.1M Phosphate Buffer Solution. Clear solubility in water with pH 3.7-4.6 at 25°C.
MP Biomedicals - 02198808 external link
(OPD Tablets)
Peroxidase substrate for EIA procedures.
Concentration: 10 mg/tablet.
Sigma Aldrich - P8287 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Sigma Aldrich - P1526 external link
Application
邻苯二胺是一种适用于 ELISA 分析的过氧化物酶底物。它可生成黄褐色的可溶性终产物,而该产物可通过分光光度法在 450nm 处读取。可用 3N HCl 或 3M H2SO4 中止 OPD 反应,并在 492nm 处读取。
Caution
储存时颜色可能变深。
Sigma Aldrich - P8806 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Sigma Aldrich - P6787 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Sigma Aldrich - P8412 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Sigma Aldrich - P6912 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Packaging
150 mg total tablet weight
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Sigma Aldrich - 78441 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Other Notes
Gas-liquid chromatography of α-ketoacids as their O-trimethylsilyl-quinoxalinol derivatives1
Sigma Aldrich - 78440 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Caution
may discolor to violet on storage
Packaging
50 g in glass bottle
Sigma Aldrich - P3804 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Packaging
16 mg total tablet weight
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Sigma Aldrich - 78449 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Sigma Aldrich - 238236 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Sigma Aldrich - P4664 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Sigma Aldrich - P6662 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Sigma Aldrich - P7288 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Sigma Aldrich - P8787 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Sigma Aldrich - P1063 external link
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

REFERENCES

REFERENCES

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PATENTS

PATENTS

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