2′,7′-Dichlorofluorescein

• Catalog No.: 35848
• CAS Number: 76-54-0
• M. F.: C20H10Cl2O5
• M. W.: 401.1964
• Purity: ≥90% (T)

SYNONYMS

• Title: 2′,7′-二氯荧光素
• IUPAC name: 2',7'-dichloro-3',6'-dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthene]-3-one
• IUPAC Traditional name: dichlorofluorescein

DATABASE IDS

• Beilstein Number: 58009
• PubChem SID: 24861978
• EC Number: 200-968-6
• MDL Number: MFCD00005047
• CAS Number: 76-54-0

PROPERTIES

• Empirical Formula (Hill Notation): C20H10Cl2O5
• Product Line: BioReagent
• Purity: ≥90% (T)
• Suitability: suitable for fluorescence
• Fluorescence: λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0
• Melting Point: 280 °C (dec.)(lit.)
• GHS Signal Word: Warning
• GHS Hazard statements: H315-H319-H335
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• GHS Precautionary statements: P261-P305 + P351 + P338
• Risk Statements: 36/37/38
• Safety Statements: 26-36
• German water hazard class: 3

DETAILS

Description (English)

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Application example:Dip reagent for the fluorimetric determination of vicinal diols, glycosides and phenols.Solution A: Saturated solution of lead tetraacetate (~2%) (Fluka-No. 15370) in glacial acetic acidSolution B: 2′,7′-Dichlorofluorescein in ethanol (0.2-1%)Mix 5 mL solution A with 5 mL solution B and bring to 200 mL with toluene, just prior to using. Solution should be freshly prepared each time. Dipping time: 10 sec.
Other usage examples: determination of carbohydrates by fluorescence densitometry after TLC; determination of H2O2 by chemiluminescence; fluorescent additive used in indirect fluorimetric detection in OTC-HPLC

Description (简体中文)

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Application example:Dip reagent for the fluorimetric determination of vicinal diols, glycosides and phenols.Solution A: Saturated solution of lead tetraacetate (~2%) (Fluka-No. 15370) in glacial acetic acidSolution B: 2',7'-Dichlorofluorescein in ethanol (0.2-1%)Mix 5 mL solution A with 5 mL solution B and bring to 200 mL with toluene, just prior to using. Solution should be freshly prepared each time. Dipping time: 10 sec.
Other usage examples: determination of carbohydrates by fluorescence densitometry after TLC; determination of H2O2 by chemiluminescence; fluorescent additive used in indirect fluorimetric detection in OTC-HPLC