Urea

• Catalog No.: 51456
• CAS Number: 57-13-6
• M. F.: CH4N2O
• M. W.: 60.05526
• Purity: ≥99.5% (T)

SYNONYMS

• Title: 尿素
• IUPAC name: urea
• IUPAC Traditional name: urea
• Synonyms: Carbamide; 碳酰胺; 碳酰二胺; Carbonyldiamide

DATABASE IDS

• EC Number: 200-315-5
• PubChem SID: 24873567
• MDL Number: MFCD00008022
• Beilstein Number: 635724
• CAS Number: 57-13-6

PROPERTIES

• Antion Traces: chloride (Cl-): ≤5 mg/kg
• Antion Traces: sulfate (SO42-): ≤10 mg/kg
• Cation Traces: Al: ≤5 mg/kg
• Cation Traces: As: ≤0.1 mg/kg
• Cation Traces: Ba: ≤5 mg/kg
• Cation Traces: Bi: ≤5 mg/kg
• Cation Traces: Ca: ≤10 mg/kg
• Cation Traces: Cd: ≤5 mg/kg
• Cation Traces: Co: ≤5 mg/kg
• Cation Traces: Cr: ≤5 mg/kg
• Cation Traces: Cu: ≤5 mg/kg
• Cation Traces: Fe: ≤5 mg/kg
• Cation Traces: K: ≤50 mg/kg
• Cation Traces: Li: ≤5 mg/kg
• Cation Traces: Mg: ≤5 mg/kg
• Cation Traces: Mn: ≤5 mg/kg
• Cation Traces: Mo: ≤5 mg/kg
• Cation Traces: Na: ≤50 mg/kg
• Cation Traces: Ni: ≤5 mg/kg
• Cation Traces: Pb: ≤5 mg/kg
• Cation Traces: Sr: ≤5 mg/kg
• Cation Traces: Zn: ≤5 mg/kg
• Grade: for molecular biology
• Ignition Residue: ≤0.01% (as SO4)
• Impurities: DNases, none detected
• Impurities: RNases, none detected
• Impurities: insoluble matter, passes filter test
• Impurities: phosphatases, none detected
• Impurities: proteases, none detected
• Impurities: ≤0.1% biuret
• Impurities: <0.005% formaldehyde (HCHO)
• λ: 8 M in H2O
• Linear Formula: NH2CONH2
• Loss on Drying: ≤0.1% loss on drying, 20 °C (HV)
• Product Line: BioUltra
• Purity: ≥99.5% (T)
• Gene Information: human ... CA1(759), CA2(760)rat ... Ppm1a(24666)
• Absorption Wavelength: λ: 260 nm Amax: ≤0.03
• Absorption Wavelength: λ: 280 nm Amax: ≤0.02
• Density: 1.335 g/mL at 25 °C(lit.)
• Melting Point: 132-135 °C(lit.)
• pH: 8.0-10.0 (20 °C, 8 M in H2O)
• Solubility: H2O: soluble8 M at 20 °C, clear, colorless
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• RTECS: YR6250000
• German water hazard class: 1

DETAILS

Description (English)

Application
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
Other Notes
Denaturing reagent1; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides2,3,4

Description (简体中文)

Application
用于蛋白质变性以及作为不溶性或变性蛋白的温和增溶剂。可用于从已由 6M 盐酸胍变性的样品(如包涵体)复性蛋白质。可与盐酸胍和二硫苏糖醇 (DTT) 一起用于复性变性蛋白以恢复其天然或活性形式。
Other Notes
Denaturing reagent1; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides2,3,4