Guanidine hydrochloride

• Catalog No.: 50935
• CAS Number: 50-01-1
• M. F.: CH6ClN3
• M. W.: 95.53144
• Purity: ≥99.5% (AT)

SYNONYMS

• Title: 胍 盐酸盐
• IUPAC name: guanidine hydrochloride
• IUPAC Traditional name: guanidine hydrochloride
• Synonyms: 盐酸胍; Guanidinium chloride; Aminomethanamidine hydrochloride; 亚氨脲 盐酸盐; Aminoformamidine hydrochloride; 亚氨基脲 盐酸盐

DATABASE IDS

• PubChem SID: 24873454
• EC Number: 200-002-3
• MDL Number: MFCD00013026
• CAS Number: 50-01-1
• Beilstein Number: 3591990

PROPERTIES

• Antion Traces: sulfate (SO42-): ≤50 mg/kg
• Cation Traces: Al: ≤5 mg/kg
• Cation Traces: As: ≤0.1 mg/kg
• Cation Traces: Ba: ≤5 mg/kg
• Cation Traces: Bi: ≤5 mg/kg
• Cation Traces: Ca: ≤10 mg/kg
• Cation Traces: Cd: ≤5 mg/kg
• Cation Traces: Co: ≤5 mg/kg
• Cation Traces: Cr: ≤5 mg/kg
• Cation Traces: Cu: ≤5 mg/kg
• Cation Traces: Fe: ≤5 mg/kg
• Cation Traces: K: ≤50 mg/kg
• Cation Traces: Li: ≤5 mg/kg
• Cation Traces: Mg: ≤5 mg/kg
• Cation Traces: Mn: ≤5 mg/kg
• Cation Traces: Mo: ≤5 mg/kg
• Cation Traces: Na: ≤50 mg/kg
• Cation Traces: Ni: ≤5 mg/kg
• Cation Traces: Pb: ≤5 mg/kg
• Cation Traces: Sr: ≤5 mg/kg
• Cation Traces: Zn: ≤5 mg/kg
• Ignition Residue: ≤0.05% (as SO4)
• Impurities: insoluble matter, passes filter test
• λ: 6 M in H2O
• Linear Formula: NH2C(=NH)NH2 · HCl
• Loss on Drying: ≤0.05% loss on drying, 20 °C (HV)
• Product Line: BioUltra
• Purity: ≥99.5% (AT)
• Absorption Wavelength: λ: 260 nm Amax: 0.03
• Absorption Wavelength: λ: 280 nm Amax: 0.02
• Melting Point: 180-185 °C(lit.)
• pH: 4.5-6.0 (25 °C, 6 M in H2O)
• Solubility: H2O: soluble6 M at 20 °C, clear, colorless
• GHS Signal Word: Warning
• GHS Hazard statements: H302-H315-H319
• European Hazard Symbols: Harmful (Xn)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves
• GHS Precautionary statements: P305 + P351 + P338
• Risk Statements: 22-36/38
• Safety Statements: 22
• German water hazard class: 2

DETAILS

Description (English)

Application
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.
Other Notes
In the extraction and fractionation by cesium sulfate density gradient centrifugation of bovine nasal cartilage proteoglycan1; Induces subunit dissociation and unfolding of bovine liver glutamate dehydrogenase2; Dissociation of apolipoproteins of insect lipophorin3; Denaturation curve4.

Description (简体中文)

Application
强效离液剂，用于蛋白质的变性以及后续复性。该强效变性剂可溶解不溶性或变性的蛋白质，如包涵体。可用作使蛋白质或酶复性为活性形式的第一步骤。可能还需要脲和二硫苏糖醇 (DTT)。
Other Notes
In the extraction and fractionation by cesium sulfate density gradient centrifugation of bovine nasal cartilage proteoglycan1; Induces subunit dissociation and unfolding of bovine liver glutamate dehydrogenase2; Dissociation of apolipoproteins of insect lipophorin3; Denaturation curve4.