Frequently Asked Questions
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Analysis Note
Molecular weight based on viscosity.
Application
Recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.
分子量 >30,000 的聚赖氨酸适用于促进细胞粘附到固体基质。
Other Notes
This product has a higher binding site density, but is more viscous than P7280. It is a poly-D-lysine that can be used with cells that digest poly-L-lysine polymers.
For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.
General description
Poly-lysine polymers are useful molecules to prepare surfaces for cell attachment. Poly-lysine polymers are available in L and D conformations. Poly-L-lysine polymers can be digested by some cell types, whereas poly-D-lysines are stable. Poly-lysine polymers from 70 to 300 kDa provide sufficient attachment site density for good cell adherence. However, higher molecular weight poly-lysine polymers are more viscous and difficult to work with.The choice of poly-lysine polymer for a specific cell type and application should account for the susceptibility of the polymer to digestion, the required attachment site density and the difficulty in handling. |