5-Bromo-4-chloro-3-indolyl phosphate disodium salt

• Catalog No.: B1026
• CAS Number: 102185-33-1
• M. F.: C8H4BrClNNa2O4P
• M. W.: 370.431961
• Purity: ≥98%

SYNONYMS

• IUPAC name: disodium 5-bromo-4-chloro-1H-indol-3-yl phosphate
• IUPAC Traditional name: disodium 5-bromo-4-chloro-1H-indol-3-yl phosphate
• Synonyms: BCIP®; X-phosphate disodium salt

DATABASE IDS

• MDL Number: MFCD00036757
• CAS Number: 102185-33-1

PROPERTIES

• Foreign Activity: Protease, none detected
• Grade: for molecular biology
• Purity: ≥98%
• Apperance: powder
• Solubility: H2O: soluble20 mg/mL
• Solubility: DMF: insoluble
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (简体中文)

Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
法律信息
BCIP 注册商标 Sigma-Aldrich Co. LLC