disodium 5-bromo-4-chloro-1H-indol-3-yl phosphate

• ChemBase ID: 102949
• Molecular Formular: C8H4BrClNNa2O4P
• Molecular Mass: 370.431961
• Monoisotopic Mass: 368.85452249
• SMILES: [Na+].[Na+].[O-]P(=O)([O-])Oc1c[nH]c2c1c(Cl)c(Br)cc2
• Canonical SMILES: Clc1c(Br)ccc2c1c(c[nH]2)OP(=O)([O-])[O-].[Na+].[Na+]
• InChI: InChI=1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2
• InChIKey: OAZUOCJOEUNDEK-UHFFFAOYSA-L

NAMES AND DATABASE IDS

Names

• IUPAC name: disodium 5-bromo-4-chloro-1H-indol-3-yl phosphate
• IUPAC Traditional name: dipotassium 5-bromo-4-chloro-1H-indol-3-yl phosphate; disodium 5-bromo-4-chloro-1H-indol-3-yl phosphate
• Synonyms: BCIP; X-phosphate; 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE DISODIUM SALT; BCIP®; X-phosphate disodium salt; 5-Bromo-4-chloro-3-indolyl phosphate disodium salt; 5-溴-4-氯-3-吲哚磷酸 二钠盐

Database IDs

• CAS Number: 102185-33-1
• MDL Number: MFCD00036757
• PubChem SID: 162089603
• PubChem CID: 6097197

CALCULATED PROPERTIES

• Acid pKa: 1.726015
• H Acceptors: 3
• H Donor: 1
• LogD (pH = 5.5): 0.10124553
• LogD (pH = 7.4): -0.6621499
• Log P: 2.4873745
• Molar Refractivity: 60.1825 cm^3
• Polarizability: 25.167912 Å^3
• Polar Surface Area: 88.21 Å^2
• Rotatable Bonds: 2
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: DMF: insoluble; H2O: soluble20 mg/mL
• Apperance: powder
• Melting Point: > 300°C

Safety Information

• Storage Condition: 0°C, Desiccate, Protect from light; -20°C, Desiccate, Protect from light
• German water hazard class: 3
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C

Product Information

• Purity: >98%; ≥98%; ≥98.0% (HPLC); 99%
• Grade: for molecular biology
• Foreign Activity: Protease, none detected
• Linear Formula: C8H4BrClNO4P · 2Na

DETAILS

MP Biomedicals - 02150042

Substrate for alkaline phosphatase
Purity: 99%

MP Biomedicals - 02193989

Molecular Biology Reagent
Disodium Salt
Purity: >98%
Chromogenic substrate for alkaline phosphatase in ELISA.

Sigma Aldrich - B1026

Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
法律信息
BCIP 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 16668

Other Notes
For visualizing antigenic proteins immobilized on nitrocellulose paper1,2
Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC