formamide

• ChemBase ID: 103273
• Molecular Formular: CH3NO
• Molecular Mass: 45.04062
• Monoisotopic Mass: 45.02146372
• SMILES: NC=O
• Canonical SMILES: NC=O
• InChI: InChI=1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChIKey: ZHNUHDYFZUAESO-UHFFFAOYSA-N

NAMES AND DATABASE IDS

Names

• IUPAC name: formamide
• IUPAC Traditional name: formamide
• Synonyms: Formamide, Spectrophotometric Grade; Formamide, ACS; HYDRANAL®-Formamide dry; Formamide solution; Carbamaldehyde; Methanamide; Formic amide; FORMAMIDE; Formamide; Formamide; Amide C1; 甲酰胺, 光谱级; 甲酰胺, ACS; 甲酰胺; HYDRANAL®-无水甲酰胺; 甲酰胺 溶液; C1 酰胺; 氨基甲醛; 甲酰胺

Database IDs

• CAS Number: 75-12-7
• EC Number: 200-842-0
• MDL Number: MFCD00007941
• Beilstein Number: 505995
• Merck Index: 144237
• PubChem SID: 24882117; 24882076; 162090353; 24871294; 24853220; 24871292; 24894943; 24894985; 24882105; 24879220; 24857817; 24861472; 24894893
• PubChem CID: 713
• CHEBI ID: 48431
• CHEMBL: 266160
• Chemspider ID: 693
• KEGG ID: C00488
• Wikipedia Title: Formamide

CALCULATED PROPERTIES

• Acid pKa: 16.66931
• H Acceptors: 1
• H Donor: 1
• LogD (pH = 5.5): -1.0789874
• LogD (pH = 7.4): -1.078987
• Log P: -1.078987
• Molar Refractivity: 9.9754 cm^3
• Polarizability: 3.849606 Å^3
• Polar Surface Area: 43.09 Å^2
• Rotatable Bonds: 0
• Lipinski's Rule of Five: true

PROPERTIES

Physical Property

• Solubility: H2O: soluble10 M at 20 °C, clear, colorless; Miscible in water
• Apperance: APHA: ≤10; clear, colorless liquid; Liquid
• Melting Point: 2 - 3°C; 2 °C; 2°C; 2-3 °C(lit.)
• Boiling Point: 210 °C; 210 °C(lit.); 210°C; 210°C
• Flash Point: 150 °C; 150°C(302°F); 154 °C (closed cup); 154°C(310°F); 175 °C (open cup and DIN 51584); 185 °F; 188.6 °F; 302 °F; 85 °C; 87 °C
• Auto Ignition Point: > 500 Grad C (DIN 51794); 932 °F
• Density: 1.132 g/mL at 20 °C; 1.133 g/cm^3; 1.133 g/cm^3 at 20 °C; 1.134; 1.134 g/mL at 25 °C(lit.)
• Refractive Index: 1.4475; n20/D 1.447; n20/D 1.447(lit.)
• Vapor Pressure: .08 hPa at 20 °C; 0.08 mmHg ( 20 °C); 0.08 mmHg at 20 °C; 30 mmHg ( 129 °C)
• Vapor Density: 1.55 (vs air); 1.56 (air = 1)
• Absorption Wavelength: λ: 260 nm Amax: ≤0.4; λ: 275 nm Amax: 1.00; λ: 280 nm Amax: ≤0.1; λ: 280 nm Amax: 0.20; λ: 300 nm Amax: 0.05; λ: 360 nm Amax: 0.02; λ: 400 nm Amax: 0.01
• pKa: 23.5 (in DMSO)
• Transition Temperature: freezing point 2.0-3.0 °C; solidification point 2-3 °C; transition temp 2.0-3.0 °C
• pH: 7-9 (25 °C, 1 M in H2O)

Safety Information

• Storage Condition: Room Temperature (15-30°C)
• Storage Warning: Hygroscopic
• RTECS: LQ0525000
• European Hazard Symbols: Nature polluting (N); Toxic (T); Harmful (Xn)
• German water hazard class: 1; 2
• Risk Statements: 61; R:36-58-68
• Safety Statements: 53-45; S:26-29-36/37/39-53-61
• TSCA Listed: 是
• GHS Pictograms: GHS05; GHS06; GHS08
• GHS Signal Word: Danger
• NFPA704:
  

  1

  2

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• GHS Hazard statements: H318-H331-H340-H360D; H360; H360D
• GHS Precautionary statements: P201-P261-P280-P305 + P351 + P338-P311; P201-P308 + P313; P281-P201-P202-P308+P313-P405-P501A
• Personal Protective Equipment: Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
• Storage Temperature: 2-8°C

Product Information

• Purity: ≥98.0% (T); ≥98.5%; ≥99%; ≥99% (GC); ≥99.0%; ≥99.0% (GC); ≥99.5%; ≥99.5% (GC); ≥99.5% (GC/T); ≥99.5% (T); 98%; 99%; 99.5+%; 99+%
• Concentration: 45% in DMSO-d6 (99.9 atom % D); 90% in DMSO-d6 (99.9 atom % D)
• Grade: ACS reagent; for molecular biology; JIS special grade; NMR reference standard; puriss. p.a.; purum; reagent grade; ReagentPlus®; SAJ first grade; spectrophotometric grade
• Suitability: complies for miscibility; in accordance for miscibility; suitable for nucleic acid hybridization; use tested
• Ignition Residue: <0.1%; ≤0.1%
• Impurities: <0.0005% Phosphorus (P); <0.030% water; ≤0.02% free acid (as HCOOH); ≤0.02% water (Karl Fischer); ≤0.1% water; DNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; RNases, none detected
• Cation Traces: Ag: ≤5 mg/kg; Al: <0.0005%; Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: <0.0005%; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: <0.0005%; Cu: ≤1 mg/kg; Fe: <0.0005%; Fe: ≤1 mg/kg; K: <0.005%; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: <0.0005%; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Na: <0.005%; Na: ≤20 mg/kg; NH4+: <0.05%; NH4+: ≤1000 mg/kg; Ni: ≤1 mg/kg; Pb: <0.001%; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Tl: ≤5 mg/kg; Zn: <0.0005%; Zn: ≤1 mg/kg
• Antion Traces: chloride (Cl-): <0.05%; chloride (Cl-): ≤1 mg/kg; sulfate (SO42-): <0.05%; sulfate (SO42-): ≤10 mg/kg
• NMR tube O.D. × L: 3 mm × 8 in.; 5 mm × 8 in.
• λ: 10 M in H2O; H2O reference
• Compatibility: Solvent for KF titration
• Product Line: BioUltra
• NMR tube wall thickness: 0.24 mm , ultra-thin wall
• NMR tube O.D.: 10 mm; 5 mm
• Linear Formula: HCONH2
• Empirical Formula (Hill Notation): CH3NO

DETAILS

MP Biomedicals - 02151160

Purity: 98%
1 ml = ~1.13 g

Sigma Aldrich - 47671

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Other Notes
For the preparation of formamide denaturing polyacrylamide gels1; In situ hybridization on human chromosomes2

Sigma Aldrich - F7508

Application
甲酰胺可使核酸双螺旋不稳定，通常可以 50% 的浓度，用于需要较低杂交温度的杂交实验。

Sigma Aldrich - F9037

Application
甲酰胺可使核酸双螺旋不稳定，通常可以 50% 的浓度，用于需要较低杂交温度的杂交实验。
包装
Packaged under nitrogen in amber glass bottles.
Preparation Note
Deionized

Sigma Aldrich - F4761

Application
甲酰胺可使核酸双螺旋不稳定，通常可以 50% 的浓度，用于需要较低杂交温度的杂交实验。

Sigma Aldrich - F5786

Application
甲酰胺可使核酸双螺旋不稳定，通常可以 50% 的浓度，用于需要较低杂交温度的杂交实验。

Sigma Aldrich - 736201

Features and Benefits
15N sensitivity

Sigma Aldrich - 733784

Features and Benefits
15N sensitivity

Sigma Aldrich - 185906

Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Packaging
2 L in glass bottle
500 mL in glass bottle

Sigma Aldrich - 93572

Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Sigma Aldrich - 613339

Features and Benefits
15N sensitivity

Sigma Aldrich - 611891

Features and Benefits
15N sensitivity

Sigma Aldrich - 551384

Features and Benefits
15N sensitivity

Sigma Aldrich - 34724

Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
General description
Visit our HYDRANAL center to learn more.
Legal Information
HYDRANAL is a registered trademark of Sigma-Aldrich Laborchemikalien GmbH
Protocols & Applications
Water Determination in Chocolate and Cocoa

Sigma Aldrich - 612707

Features and Benefits
15N sensitivity

Sigma Aldrich - 11-0753

Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Preparation Note
deionized
Suitability
for biological purposes

Sigma Aldrich - 47680

Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Sigma Aldrich - 295876

Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Packaging
1, 2 L in glass bottle

Sigma Aldrich - 11-0740

Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Sigma Aldrich - 11-0755

Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Suitability
for biological purposes

Sigma Aldrich - 221198

Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Packaging
1, 4×4 L in glass bottle
100, 500 mL in glass bottle

Sigma Aldrich - F7503

Application
甲酰胺可使核酸双螺旋不稳定，通常可以 50% 的浓度，用于需要较低杂交温度的杂交实验。
包装
1 L in glass bottle
2.5, 4 L in poly bottle
5, 100, 250, 500 mL in glass bottle
法律信息
ReagentPlus 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 11-0750

Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Sigma Aldrich - 47670

Application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.