Nitrotetrazolium Blue chloride

• Catalog No.: N6639
• CAS Number: 298-83-9
• M. F.: C40H30Cl2N10O6
• M. W.: 817.6356

SYNONYMS

• IUPAC name: 2-(2-methoxy-4-{3-methoxy-4-[3-(4-nitrophenyl)-5-phenyl-3H-1,2λ^5,3,4-tetrazol-2-ylium-2-yl]phenyl}phenyl)-3-(4-nitrophenyl)-5-phenyl-3H-1,2λ^5,3,4-tetrazol-2-ylium dichloride
• IUPAC Traditional name: nitro blue tetrazolium(2+) dichloride
• Synonyms: NBT; 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; Nitro BT; 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]

DATABASE IDS

• CAS Number: 298-83-9
• Beilstein Number: 4115923
• MDL Number: MFCD00012159
• EC Number: 206-067-4
• PubChem SID: 24897786

PROPERTIES

• Grade: for microscopy
• Grade: for molecular biology
• Linear Formula: C40H30N10O6 · 2Cl
• Apperance: powder
• Solubility: H2O: soluble10 mg/mL (A stock solution at 10mg/ml in water is stable for 1-2 weeks in the dark at 0-4°C.)
• Solubility: 2-methoxyethanol: soluble20 mg/mL
• Solubility: ethanol: soluble5 mg/mL
• GHS Signal Word: Warning
• GHS Hazard statements: H302
• European Hazard Symbols: Harmful (Xn)
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Risk Statements: 22
• RTECS: XF8045000
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Application
In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques.
Packaging
1 g in glass bottle
25, 50, 250 mg in glass bottle

Description (简体中文)

Application
In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques.
包装
1 g in glass bottle
25, 50, 250 mg in glass bottle