Demecolcine

• Catalog No.: 27645
• CAS Number: 477-30-5
• M. F.: C21H25NO5
• M. W.: 371.4269
• Purity: ≥98.0% (sum of enantiomers, HPLC)

SYNONYMS

• Title: 地美可辛
• IUPAC name: (10S)-3,4,5,14-tetramethoxy-10-(methylamino)tricyclo[9.5.0.0^2,^7]hexadeca-1(16),2,4,6,11,14-hexaen-13-one
• IUPAC Traditional name: colcemid
• Synonyms: N-Deacetyl-N-methylcolchicine; N-脱乙酰-N-甲基秋水仙碱

DATABASE IDS

• PubChem SID: 24856707
• Beilstein Number: 2822892
• EC Number: 207-514-6
• CAS Number: 477-30-5
• MDL Number: MFCD00075459

PROPERTIES

• Empirical Formula (Hill Notation): C21H25NO5
• Purity: ≥98.0% (sum of enantiomers, HPLC)
• GHS Signal Word: Danger
• GHS Hazard statements: H300
• European Hazard Symbols: Toxic (T)
• Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
• GHS Precautionary statements: P264-P301 + P310
• RID/ADR: UN 2811 6.1/PG 2
• Risk Statements: 25
• RTECS: GH0800000
• Safety Statements: 22-24/25-45
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Biochem/physiol Actions
Depolymerizes microtubules; blocks mitosis at metaphase. Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.
Other Notes
Binds tubulin hence interfering with microtubule-dependent cell function1

Description (简体中文)

Biochem/physiol Actions
解聚微管；在四分体时期阻断有丝分裂。 常用于染色体核型分析与细胞周期研究，以增加停留在细胞周期特定阶段的有丝分裂细胞的产量。可采用多种途径达到该目的，其中最常用的便是使用如地美可辛之类的细胞周期同步化试剂。地美可辛能使有丝分裂细胞或 G1 和 S 期的同步化细胞停留在四分体时期，同时也不会对其生化反应造成明显影响。通常用地美可辛处理白细胞以使细胞停留在四分体时期。
Other Notes
Binds tubulin hence interfering with microtubule-dependent cell function1