Ammonium acetate

• Catalog No.: 09688
• CAS Number: 631-61-8
• M. F.: C2H7NO2
• M. W.: 77.08248
• Purity: ≥99.0% (calc. based on dry substance, T)

SYNONYMS

• Title: 乙酸铵
• IUPAC name: acetic acid amine
• IUPAC Traditional name: acetic acid amine

DATABASE IDS

• MDL Number: MFCD00013066
• EC Number: 211-162-9
• CAS Number: 631-61-8
• Beilstein Number: 4186741
• PubChem SID: 24846389

PROPERTIES

• Antion Traces: chloride (Cl-): ≤5 mg/kg
• Antion Traces: nitrate (NO3-): ≤10 mg/kg
• Antion Traces: sulfate (SO42-): ≤10 mg/kg
• Cation Traces: Al: ≤5 mg/kg
• Cation Traces: As: ≤0.1 mg/kg
• Cation Traces: Ba: ≤5 mg/kg
• Cation Traces: Bi: ≤5 mg/kg
• Cation Traces: Ca: ≤10 mg/kg
• Cation Traces: Cd: ≤5 mg/kg
• Cation Traces: Co: ≤5 mg/kg
• Cation Traces: Cr: ≤5 mg/kg
• Cation Traces: Cu: ≤5 mg/kg
• Cation Traces: Fe: ≤2 mg/kg
• Cation Traces: K: ≤50 mg/kg
• Cation Traces: Li: ≤5 mg/kg
• Cation Traces: Mg: ≤5 mg/kg
• Cation Traces: Mn: ≤5 mg/kg
• Cation Traces: Mo: ≤5 mg/kg
• Cation Traces: Na: ≤50 mg/kg
• Cation Traces: Ni: ≤5 mg/kg
• Cation Traces: Pb: ≤5 mg/kg
• Cation Traces: Sr: ≤5 mg/kg
• Cation Traces: Zn: ≤5 mg/kg
• Grade: for molecular biology
• Ignition Residue: ≤0.01% (as SO4)
• Impurities: DNases, none detected
• Impurities: RNases, none detected
• Impurities: insoluble matter, passes filter test
• Impurities: phosphatases, none detected
• Impurities: proteases, none detected
• Impurities: ≤2% water
• λ: 1 M in H2O
• Linear Formula: CH3CO2NH4
• Product Line: BioUltra
• Purity: ≥99.0% (calc. based on dry substance, T)
• Absorption Wavelength: λ: 260 nm Amax: ≤0.015
• Absorption Wavelength: λ: 280 nm Amax: ≤0.010
• Apperance: solid
• Melting Point: 110-112 °C (dec.)(lit.)
• pH: 6.5-7.5 (25 °C, 1 M in H2O)
• Solubility: H2O: soluble1 M at 20 °C, clear, colorless
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• RTECS: AF3675000
• German water hazard class: 1

DETAILS

Description (English)

Other Notes
Alternative to sodium acetate in ethanol precipitation of nucleic acids. Prevents precipitation of deoxyribonucleoside triphosphates.; Useful for removal of untreated triphosphates from the products of reverse transcriptase, DNA polymerase or terminal polymerase-catalyzed reactions1.
Application
Ammonium acetate can be used to study molecular biology, reagents, protein structural analysis, proteomics and x-ray crystallography. Ammonium acetate has been used in a study to develop an easy, rapid, low-cost and effective cetyltrimethylammonium bromide (CTAB)-ammonium acetate method for high-quality RNA isolation from polysaccharide- and polyphenol-rich cotton tissues. Ammonium acetate has also been used in a study to develop and validate a simple and rapid high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method for the determination of 4-p-anisamidobutyric acid (ABA; or N-anysoyl-γ-aminobutiryc acid, N-anisoyl-GABA), a major active metabolite of aniracetam, in human plasma.

Description (简体中文)

Other Notes
Alternative to sodium acetate in ethanol precipitation of nucleic acids. Prevents precipitation of deoxyribonucleoside triphosphates.; Useful for removal of untreated triphosphates from the products of reverse transcriptase, DNA polymerase or terminal polymerase-catalyzed reactions1.
Application
Ammonium acetate can be used to study molecular biology, reagents, protein structural analysis, proteomics and x-ray crystallography. Ammonium acetate has been used in a study to develop an easy, rapid, low-cost and effective cetyltrimethylammonium bromide (CTAB)-ammonium acetate method for high-quality RNA isolation from polysaccharide- and polyphenol-rich cotton tissues. Ammonium acetate has also been used in a study to develop and validate a simple and rapid high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method for the determination of 4-p-anisamidobutyric acid (ABA; or N-anysoyl-γ-aminobutiryc acid, N-anisoyl-GABA), a major active metabolite of aniracetam, in human plasma.