Fluorescent red 630 reactive

• Catalog No.: 78361
• CAS Number: 350496-73-0
• M. F.: C40H49N3O8S
• M. W.: 731.89736
• Purity: ≥80% (coupling to amines)

SYNONYMS

• Title: 荧光红 HFG 活性剂
• IUPAC name: 2-tert-butyl-7-(diethylamino)-4-[3-(1-{6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-3,3-dimethyl-5-sulfonato-2,3-dihydro-1H-indol-2-ylidene)prop-1-en-1-yl]-1λ^4-chromen-1-ylium
• IUPAC Traditional name: 2-tert-butyl-7-(diethylamino)-4-[3-(1-{6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-3,3-dimethyl-5-sulfonatoindol-2-ylidene)prop-1-en-1-yl]-1λ^4-chromen-1-ylium
• Synonyms: Reactive fluorescent red 630; 荧光红 HFG 激活剂

DATABASE IDS

• CAS Number: 350496-73-0
• MDL Number: MFCD03456833
• PubChem SID: 24887291

PROPERTIES

• Empirical Formula (Hill Notation): C40H49N3O8S
• Grade: for fluorescence
• Purity: ≥80% (coupling to amines)
• Fluorescence: λex 620 nm; λem 650 nm in 0.1 M phosphate pH 7.0
• Solubility: DMF: soluble
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (English)

Application
Amino-reactive biolabel excited by red lasers (He/Ne; 633 nm) and diode lasers (635-650 nm). The chromophore is a bridged hemicyanine. Quantum yield is fairly low in aqueous media, but increases up to one order of magnitude if coupled to a target protein; Molar absorption 120,000 M-1 cm-1, λabs: (conjugate) 645 nm; λem: (conjugate) 660 nm
Features and Benefits
This novel hemocyanin dye is optimally excited by He-Ne laser with a wavelength of 633 nm, but may also be excited by argon or argon-krypton laser with a wavelength of 647 nm or diode lasers.Additional advantages are:High molar absorptions (120,000 M-1cm-1)Low quantum yields in nonaqueous media, greatly increased by binding to target molecules.Very strong fluorescence in solid state, which allows preparation of DNA for arrays on glass.

Description (简体中文)

Application
由红色激光（633nm 氦氖激光）和二极管激光 (635-650nm) 激活的氨基活性生物标记物。发色团为桥联半花青。水相中量子产量极低，但将其连接至目标蛋白后，量子产量增加多达一个数量级；摩尔吸光系数为 120,000M-1 cm-1，λabs：（偶联物）645nm；λem：（偶联物）660nm
Features and Benefits
这种新型血蓝蛋白染料最好用 633nm 氦氖激光激活，但也可以通过 647nm 氩激光或氩氪激光或者二极管激光激活。其他优点包括：高摩尔吸光系数 (120,000M-1cm-1)在非水介质中量子产率低，但将其键合至目标分子后，量子产率激增。固态具有极强荧光，可用于在玻璃上制备 DNA 阵列。