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Fast Green FCF_Molecular_structure_CAS_2353-45-9)
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Fast Green FCF

Catalog No. 68724 Name Sigma Aldrich
CAS Number 2353-45-9 Website http://www.sigmaaldrich.com
M. F. C37H34N2Na2O10S3 Telephone 1-800-521-8956
M. W. 808.8478 Fax
Purity ≥98.0% (HPLC) Email
Storage Chembase ID: 109627

SYNONYMS

IUPAC name
disodium 2-[(4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)(4-{ethyl[(3-sulfonatophenyl)methyl]iminiumyl}cyclohexa-2,5-dien-1-ylidene)methyl]-5-hydroxybenzene-1-sulfonate
IUPAC Traditional name
disodium 2-[(4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)(4-{ethyl[(3-sulfonatophenyl)methyl]iminio}cyclohexa-2,5-dien-1-ylidene)methyl]-5-hydroxybenzenesulfonate
Synonyms
Food green 3

DATABASE IDS

Beilstein Number 5718212
Color Index Number 42053
MDL Number MFCD00013053
CAS Number 2353-45-9
E Number E143

PROPERTIES

Grade analytical standard
Linear Formula C37H34N2O10S3Na2
Purity ≥98.0% (HPLC)
Melting Point 290 °C (dec.)(lit.)
MSDS Link Download

DETAILS

Description (English)
Application
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Description (简体中文)
Application
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

REFERENCES