N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

• Catalog No.: 03451
• CAS Number: 25952-53-8
• M. F.: C8H18ClN3
• M. W.: 191.70162

SYNONYMS

• Title: 1-(3-二甲氨基丙基)-3-乙基碳二亚胺 盐酸盐
• IUPAC name: (3-{[(ethylimino)methylidene]amino}propyl)dimethylamine hydrochloride
• IUPAC Traditional name: (3-{[(ethylimino)methylidene]amino}propyl)dimethylamine hydrochloride
• Synonyms: WSC hydrochloride; EDC hydrochloride; EDAC; N-乙基-N′-(3-二甲基氨丙基)碳二亚胺 盐酸盐; EDC 盐酸盐; WSC 盐酸盐; N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride

DATABASE IDS

• Beilstein Number: 5764110
• EC Number: 247-361-2
• CAS Number: 25952-53-8
• MDL Number: MFCD00012503

PROPERTIES

• Empirical Formula (Hill Notation): C8H17N3 · HCl
• Grade: for protein sequence analysis
• Melting Point: 110-115 °C(lit.)
• GHS Signal Word: Danger
• GHS Hazard statements: H315-H318-H335
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• GHS Precautionary statements: P261-P280-P305 + P351 + P338
• Risk Statements: 37/38-41
• RTECS: FF2200000
• Safety Statements: 26-36/37/39
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (English)

Other Notes
Peptide activation and coupling to solid supports under conditions favouring selective activation of the terminal carboxyl group1
Biochem/physiol Actions
Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

Description (简体中文)

Other Notes
在有利于选择性活化末端羧基1的情况下，活化肽并偶联到固体载体上
Biochem/physiol Actions
水溶性缩合剂。常用作酰胺与伯胺键合的羧基活化剂。此外，它可与磷酸基团反应。可用于例如肽合成；蛋白质和核酸的交联；以及免疫交联物的制备。通常，在无缓冲液的条件下，其工作 pH 范围为 4.0-6.0。应特别避免胺和羧酸盐缓冲液。