Fluorescent red 635 reactive

• Catalog No.: 39779
• CAS Number: 350496-77-4
• M. F.: C42H49N3O8S
• M. W.: 755.91876

SYNONYMS

• IUPAC name: 2-(3-{4-tert-butyl-3-oxa-13-azatetracyclo[7.7.1.0^2,^7.0^1^3,^1^7]heptadeca-1(17),2(7),4,8-tetraen-6-ylidene}prop-1-en-1-yl)-1-{6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
• IUPAC Traditional name: 2-(3-{4-tert-butyl-3-oxa-13-azatetracyclo[7.7.1.0^2,^7.0^1^3,^1^7]heptadeca-1(17),2(7),4,8-tetraen-6-ylidene}prop-1-en-1-yl)-1-{6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-3,3-dimethylindol-1-ium-5-sulfonate
• Synonyms: 2-[3-(11-tert.-Butylpyrano[3,2-g]pyrido[3,2,1-ij]quinolin-9-ylidene)-1-propenyl]-1-[5-(succinimidooxycarbonyl)-pentyl]-3,3-dimethylindolio-5-sulfonate

DATABASE IDS

• CAS Number: 350496-77-4
• MDL Number: MFCD03099336
• PubChem SID: 24864707

PROPERTIES

• Empirical Formula (Hill Notation): C42H49N3O8S
• Grade: for fluorescence
• Fluorescence: λex 635 nm; λem 665 nm in 0.1 M phosphate pH 7.0 (non-covalent binding to BSA enhances fluorescence emission)
• Solubility: DMF: soluble
• GHS Signal Word: Warning
• GHS Hazard statements: H315-H319-H335
• European Hazard Symbols: Irritant (Xi)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• GHS Precautionary statements: P261-P305 + P351 + P338
• Risk Statements: 36/37/38
• Safety Statements: 26
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Application
Amino-reactive biolabel excited by red lasers (He-Ne; 633 nm) and diode lasers (635-650 nm). Molar absorption 200′000 1/M cm, abs: (conjugate) 650 nm, em: (conjugate) 670 nm, QY is fairly low in aqueous media, but up to 0.4 for conjugates.
Features and Benefits
Hemicyanine dye Fluorescent red 630 match excitation by He-Ne laser line at 633 nm, Argon or Argon-Krypton laser lines at 647 nm and diode lasers (635-650 nm). BenefitsIdeal match to common excitation light sourcesHigh molar absorption (200.000 M-1cm-1) Fairly low quantum yield if dissolved inaqueous media, but very strong increase by coupling to target molecule.Superior solid state fluorescence making them certainly useful to label DNA for preparation of glass arrays
General description
Fluorescent red NHS is an aminoreactive fluorescent biolabel designed for excitation by red lasers (He/Ne; 633 nm) and diode lasers (635 - 650 nm). It can be used for covalent coupling to proteins and other biomolecules containing primary amino groups like aminomodified DNA-oligomers and aminomodified biotin. The chromophore responsible for absoprtion maximum is based on a bridged hemicyanine.Fluorescent red 635-NHS exhibits a fairly low fluorescence quantum yield if dissolved in aqueous media, but after coupling to a target signal intensity can rise up to one order of magnitude as demonstrated for protein conjugates and labeled oligomers. Together with the quantum yield the fluorescence lifetime increases drastically.Within the conjugate absorption of the label shifts slightly to the red while position and shape of the emission spectrum remain nearly unchanged.

Description (简体中文)

Application
Amino-reactive biolabel excited by red lasers (He-Ne; 633 nm) and diode lasers (635-650 nm). Molar absorption 200′000 1/M cm, abs: (conjugate) 650 nm, em: (conjugate) 670 nm, QY is fairly low in aqueous media, but up to 0.4 for conjugates.
Features and Benefits
Hemicyanine dye Fluorescent red 630 match excitation by He-Ne laser line at 633 nm, Argon or Argon-Krypton laser lines at 647 nm and diode lasers (635-650 nm). BenefitsIdeal match to common excitation light sourcesHigh molar absorption (200.000 M-1cm-1) Fairly low quantum yield if dissolved inaqueous media, but very strong increase by coupling to target molecule.Superior solid state fluorescence making them certainly useful to label DNA for preparation of glass arrays
General description
Fluorescent red NHS is an aminoreactive fluorescent biolabel designed for excitation by red lasers (He/Ne; 633 nm) and diode lasers (635 - 650 nm). It can be used for covalent coupling to proteins and other biomolecules containing primary amino groups like aminomodified DNA-oligomers and aminomodified biotin. The chromophore responsible for absoprtion maximum is based on a bridged hemicyanine.Fluorescent red 635-NHS exhibits a fairly low fluorescence quantum yield if dissolved in aqueous media, but after coupling to a target signal intensity can rise up to one order of magnitude as demonstrated for protein conjugates and labeled oligomers. Together with the quantum yield the fluorescence lifetime increases drastically.Within the conjugate absorption of the label shifts slightly to the red while position and shape of the emission spectrum remain nearly unchanged.