Diethyl pyrocarbonate

• Catalog No.: 32490
• CAS Number: 1609-47-8
• M. F.: C6H10O5
• M. W.: 162.1406
• Purity: ≥97.0% (NT)

SYNONYMS

• Title: 焦碳酸二乙酯
• IUPAC name: diethyl dicarbonate
• IUPAC Traditional name: diethyl pyrocarbonate
• Synonyms: Diethyl dicarbonate; DEP; 焦炭酸二乙酯; 氧二甲酸二乙酯; 重碳酸二乙酯; Diethyl Oxydiformate; DEPC; Ethoxyformic acid anhydride

DATABASE IDS

• PubChem SID: 24859476
• EC Number: 216-542-8
• CAS Number: 1609-47-8
• Beilstein Number: 637031
• MDL Number: MFCD00009106

PROPERTIES

• Grade: purum
• Linear Formula: O(COOC2H5)2
• Purity: ≥97.0% (NT)
• Shelf Life: (limited shelf life, expiry date on the label)
• Boiling Point: 93-94 °C/18 mmHg(lit.)
• Density: 1.12 g/mL at 20 °C
• Density: 1.101 g/mL at 25 °C(lit.)
• Flash Point: 69 °C
• Flash Point: 156.2 °F
• Refractive Index: n20/D 1.398(lit.)
• Refractive Index: n20/D 1.398
• GHS Signal Word: Warning
• GHS Hazard statements: H302-H315-H319-H335
• European Hazard Symbols: Harmful (Xn)
• Personal Protective Equipment: Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
• GHS Precautionary statements: P261-P305 + P351 + P338
• Risk Statements: 22-36/37/38
• RTECS: LQ9350000
• Safety Statements: 26-36
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Application
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.1
Biochem/physiol Actions
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
Other Notes
Enzyme inhibitor: reagent for the selective chemical modification of histidine 2; Modification of nucleotides: preferential carbethoxylation of purine N-7 3

Description (简体中文)

Application
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.1
Biochem/physiol Actions
在溶液中浓度约为 0.1% (v/v) 时可使 RNase 失活，从而防止 RNA 被降解。
Other Notes
酶抑制剂：组氨酸的选择性化学改性试剂2；核苷酸的改性：嘌呤 N-7 的优先乙酯基化3