JC-1

• Catalog No.: 40882
• CAS Number: 3520-43-2
• M. F.: C25H27Cl4IN4
• M. W.: 652.22515
• Purity: ≥90% (HPCE)

SYNONYMS

• IUPAC name: 5,6-dichloro-2-{3-[(2E)-5,6-dichloro-1,3-diethyl-2,3-dihydro-1H-1,3-benzodiazol-2-ylidene]prop-1-en-1-yl}-1,3-diethyl-1H-1,3-benzodiazol-3-ium iodide
• IUPAC Traditional name: 5,6-dichloro-2-{3-[(2E)-5,6-dichloro-1,3-diethyl-1,3-benzodiazol-2-ylidene]prop-1-en-1-yl}-1,3-diethyl-1,3-benzodiazol-1-ium iodide
• Synonyms: 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide; CBIC2(3); 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide

DATABASE IDS

• MDL Number: MFCD00188475
• PubChem SID: 24865598
• CAS Number: 3520-43-2

PROPERTIES

• Empirical Formula (Hill Notation): C25H27Cl4IN4
• Purity: ≥90% (HPCE)
• Fluorescence: λex 520 nm; λem 596 nm in 0.1 M Tris pH 8.0
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• German water hazard class: 3

DETAILS

Description (English)

Application
A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density.

Description (简体中文)

Application
A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density.