DOTAP methosulfate

• Catalog No.: 90189
• CAS Number: 144189-73-1
• M. F.: C43H83NO8S
• M. W.: 774.18602

SYNONYMS

• IUPAC name: [2,3-bis(octadec-9-enoyloxy)propyl]trimethylazanium methyl sulfate
• IUPAC Traditional name: [2,3-bis(octadec-9-enoyloxy)propyl]trimethylazanium methyl sulfate(1-)
• Synonyms: Transfection reagent; N-(2,3-Dioleoyloxy-1-propyl)trimethylammonium methyl sulfate

DATABASE IDS

• MDL Number: MFCD00274414
• PubChem SID: 24889327
• CAS Number: 144189-73-1

PROPERTIES

• Empirical Formula (Hill Notation): C43H83NO8S
• Grade: for molecular biology
• Personal Protective Equipment: Eyeshields, Gloves
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Application
Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.
liposomal transfection reagent (1mg/ml)
Other Notes
Mediates transfection of mammalian cells1

Description (简体中文)

Application
Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.
liposomal transfection reagent (1mg/ml)
Other Notes
Mediates transfection of mammalian cells1