Isopropyl β-D-1-thiogalactopyranoside

• Catalog No.: 59740
• CAS Number: 367-93-1
• M. F.: C9H18O5S
• M. W.: 238.30122
• Purity: ≥99.0% (TLC)

SYNONYMS

• Title: 异丙基 β-D-1-硫代半乳糖吡喃糖苷
• IUPAC name: (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(propan-2-ylsulfanyl)oxane-3,4,5-triol
• IUPAC Traditional name: IPTG
• Synonyms: Isopropyl β-D-thiogalactoside; IPTG; 异丙基 β-D-硫代半乳糖苷

DATABASE IDS

• CAS Number: 367-93-1
• MDL Number: MFCD00063273
• PubChem SID: 24881691
• EC Number: 206-703-0
• Beilstein Number: 4631

PROPERTIES

• Description: non-ionic
• Empirical Formula (Hill Notation): C9H18O5S
• Ignition Residue: ≤0.2%
• Loss on Drying: ≤6% loss on drying
• Purity: ≥99.0% (TLC)
• Optical Rotation: [α]20/D -32.0±1.0°, c = 1% in H2O (dry matter)
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (English)

Application
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Other Notes
Non-metabolizable galactose analog.
IPTG is an inducer of β-galactosidase activity in bacteria and is used to detect lac gene activity during cloning experiments1

Description (简体中文)

Application
IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用，用于重组细菌菌落的蓝白筛选，这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用，防止 β-半乳糖苷酶编码基因 lacZ 的抑制。
Other Notes
非代谢性半乳糖类似物。
IPTG 是细菌中 β-半乳糖苷酶活性诱导剂，在克隆实验中用于检测 lac 基因的活性1