4′,6-Diamidino-2-phenylindole dihydrochloride

• Catalog No.: 32670
• CAS Number: 28718-90-3
• M. F.: C16H17Cl2N5
• M. W.: 350.24568
• Purity: ≥95.0% (HPLC)

SYNONYMS

• IUPAC name: 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride
• IUPAC Traditional name: DAPI dihydrochloride
• Synonyms: DAPI dihydrochloride; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride

DATABASE IDS

• EC Number: 249-186-7
• CAS Number: 28718-90-3
• PubChem SID: 24859612
• MDL Number: MFCD00012681
• Beilstein Number: 4894417

PROPERTIES

• Empirical Formula (Hill Notation): C16H15N5 · 2HCl
• Product Line: BioReagent
• Purity: ≥95.0% (HPLC)
• Suitability: suitable for fluorescence
• Fluorescence: λex 340 nm; λem 488 nm (nur DAPI)
• Fluorescence: λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
• Fluorescence: λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA
• Solubility: DMF: soluble
• Solubility: H2O: soluble
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• German water hazard class: 3

DETAILS

Description (English)

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Application
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Description (简体中文)

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Application
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.