bisBenzimide H 33342 trihydrochloride

• Catalog No.: 14533
• CAS Number: 23491-52-3
• M. F.: C29H35Cl3N6O
• M. W.: 589.9868
• Purity: ≥97.0% (HPLC)

SYNONYMS

• IUPAC name: 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1,4-dihydroquinazolin-2-yl]-1,4-dihydroquinazoline trihydrochloride
• IUPAC Traditional name: 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1,4-dihydroquinazolin-2-yl]-1,4-dihydroquinazoline trihydrochloride
• Synonyms: bisBenzimide; Hoechst 33342; 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride; HOE 33342

DATABASE IDS

• EC Number: 245-691-1
• MDL Number: MFCD00012678
• PubChem SID: 24848717
• Beilstein Number: 1234011
• CAS Number: 23491-52-3

PROPERTIES

• Empirical Formula (Hill Notation): C27H28N6O · 3HCl · xH2O
• Grade: for fluorescence
• Purity: ≥97.0% (HPLC)
• Fluorescence: λex 340 nm; λem 510 nm
• Fluorescence: λex 355 nm; λem 465 nm in TE buffer; DNA
• GHS Signal Word: Danger
• GHS Hazard statements: H302-H315-H318-H335
• European Hazard Symbols: Harmful (Xn)
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves
• GHS Precautionary statements: P261-P280-P305 + P351 + P338
• Risk Statements: 22-37/38
• RTECS: DT4200000
• Safety Statements: 22-36/37
• Storage Temperature: 2-8°C
• German water hazard class: 3

DETAILS

Description (English)

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.Excitation max. = 346 nmEmission max. = 460 nm
bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP.
Biochem/physiol Actions
Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.
Other Notes
Suitable for vital DNA staining of a variety of cell types1,2; As a probe of membrane permeability in mammalian cells3

Description (简体中文)

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.Excitation max. = 346 nmEmission max. = 460 nm
bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP.
Biochem/physiol Actions
Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.
Other Notes
Suitable for vital DNA staining of a variety of cell types1,2; As a probe of membrane permeability in mammalian cells3