Bis(2,2′-bipyridine)-4,4′-dicarboxybipyridine-ruthenium di(N-succinimidyl ester) bis(hexafluorophosphate)

• Catalog No.: 96632
• CAS Number: 160525-49-5
• M. F.: C40H30F12N8O8P2Ru
• M. W.: 1141.7133604

SYNONYMS

• IUPAC name: λ^2-ruthenium(2+) ion bis(2-(pyridin-2-yl)pyridine) bis(hexafluoro-λ^5-phosphanuide) 2,5-dioxopyrrolidin-1-yl 2-(4-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}pyridin-2-yl)pyridine-4-carboxylate
• IUPAC Traditional name: λ^2-ruthenium(2+) ion bis(bipyridyl) 2,5-dioxopyrrolidin-1-yl 2-(4-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}pyridin-2-yl)pyridine-4-carboxylate dihexafluorophosphate
• Synonyms: Ru(bpy)2(dcbpy-di O-Su-ester)(PF6)2

DATABASE IDS

• PubChem SID: 24890332
• CAS Number: 160525-49-5
• MDL Number: MFCD02683416

PROPERTIES

• Empirical Formula (Hill Notation): C40H30F12N8O8P2Ru
• Product Line: BioReagent
• Shipped in: dry ice
• Suitability: suitable for fluorescence
• Fluorescence: λex 458 nm; λem 647 nm in 0.1 M phosphate pH 7.0
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (English)

Application
Ruthenium-ligand belonging to a new class of long-lived fluorophores for protein hydrodynamics.
Features and Benefits
Sigma-Aldrich offers a series of reactive dyes based on ruthenium complexes for use in fluorescence polarization assays and fluorescence lifetime imaging applications. They have been designed specifically to have long fluorescence lifetimes. These dyes were first synthesized in the labs of Dr. Joseph Lakowicz at the Center for Fluorescence Microscopy. These laser-excitable metal ligand complexes have fluorescence lifetimes near 500 nanoseconds. These dyes can be used to label amines on biomolecules under mild conditions. Advantages of these dyes as protein labels include high photostability, good water solubility, a lack of dye-dye interactions and large Stokes’ shifts. In addition, the fluorescence signal of long-lived fluorophores can be gated to eliminate the emission from short-lifetime fluorophores and autofluorescence from cells and biomolecules to further improve sensitivity.

Description (简体中文)

Application
Ruthenium-ligand belonging to a new class of long-lived fluorophores for protein hydrodynamics.
Features and Benefits
Sigma-Aldrich offers a series of reactive dyes based on ruthenium complexes for use in fluorescence polarization assays and fluorescence lifetime imaging applications. They have been designed specifically to have long fluorescence lifetimes. These dyes were first synthesized in the labs of Dr. Joseph Lakowicz at the Center for Fluorescence Microscopy. These laser-excitable metal ligand complexes have fluorescence lifetimes near 500 nanoseconds. These dyes can be used to label amines on biomolecules under mild conditions. Advantages of these dyes as protein labels include high photostability, good water solubility, a lack of dye-dye interactions and large Stokes’ shifts. In addition, the fluorescence signal of long-lived fluorophores can be gated to eliminate the emission from short-lifetime fluorophores and autofluorescence from cells and biomolecules to further improve sensitivity.