Bis(2,2′-bipyridine)-(5-isothiocyanato-phenanthroline)ruthenium bis(hexafluorophosphate)

• Catalog No.: 71603
• CAS Number: 288399-07-5
• M. F.: C33H23F12N7P2RuS
• M. W.: 940.6459804

SYNONYMS

• IUPAC name: λ^2-ruthenium(2+) ion bis(2-(pyridin-2-yl)pyridine) 5-isothiocyanato-1,10-phenanthroline bis(hexafluoro-λ^5-phosphanuide)
• IUPAC Traditional name: λ^2-ruthenium(2+) ion 5-isothiocyanato-1,10-phenanthroline bis(bipyridyl) dihexafluorophosphate
• Synonyms: Ru(bpy)2(phen-5-isothiocyanate)(PF6)2

DATABASE IDS

• CAS Number: 288399-07-5
• MDL Number: MFCD02683413
• PubChem SID: 24886230

PROPERTIES

• Empirical Formula (Hill Notation): C33H23F12N7P2RuS
• Product Line: BioReagent
• Shipped in: dry ice
• Suitability: suitable for fluorescence
• Fluorescence: λex 445 nm; λem ~615 nm in 0.1 M phosphate pH 7.0
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (English)

Application
Reactive probe containing fluorescent metal-ligand complex for determination of nucleotide base sequences.
Features and Benefits
Sigma-Aldrich offers a series of reactive dyes based on ruthenium complexes for use in fluorescence polarization assays and fluorescence lifetime imaging applications. They have been designed specifically to have long fluorescence lifetimes. These dyes were first synthesized in the labs of Dr. Joseph Lakowicz at the Center for Fluorescence Microscopy. These laser-excitable metal ligand complexes have fluorescence lifetimes near 500 nanoseconds. These dyes can be used to label amines on biomolecules under mild conditions. Advantages of these dyes as protein labels include high photostability, good water solubility, a lack of dye-dye interactions and large Stokes’ shifts. In addition, the fluorescence signal of long-lived fluorophores can be gated to eliminate the emission from short-lifetime fluorophores and autofluorescence from cells and biomolecules to further improve sensitivity.

Description (简体中文)

Application
Reactive probe containing fluorescent metal-ligand complex for determination of nucleotide base sequences.
Features and Benefits
Sigma-Aldrich offers a series of reactive dyes based on ruthenium complexes for use in fluorescence polarization assays and fluorescence lifetime imaging applications. They have been designed specifically to have long fluorescence lifetimes. These dyes were first synthesized in the labs of Dr. Joseph Lakowicz at the Center for Fluorescence Microscopy. These laser-excitable metal ligand complexes have fluorescence lifetimes near 500 nanoseconds. These dyes can be used to label amines on biomolecules under mild conditions. Advantages of these dyes as protein labels include high photostability, good water solubility, a lack of dye-dye interactions and large Stokes’ shifts. In addition, the fluorescence signal of long-lived fluorophores can be gated to eliminate the emission from short-lifetime fluorophores and autofluorescence from cells and biomolecules to further improve sensitivity.