Bis(2,2′-bipyridine)-(5-aminophenanthroline)ruthenium bis(hexafluorophosphate)

• Catalog No.: 90819
• CAS Number: 84537-86-0
• M. F.: C32H25F12N7P2Ru
• M. W.: 898.5861604

SYNONYMS

• Title: 双(2,2-二吡啶)-(5-氨基邻二氮杂菲)双(六氟磷酸)钌
• IUPAC name: λ^2-ruthenium(2+) ion 1,10-phenanthrolin-5-amine bis(2-(pyridin-2-yl)pyridine) bis(hexafluoro-λ^5-phosphanuide)
• IUPAC Traditional name: λ^2-ruthenium(2+) ion 1,10-phenanthrolin-5-amine bis(bipyridyl) dihexafluorophosphate
• Synonyms: Ru(bpy)2(phen-5-NH2)(PF6)2

DATABASE IDS

• PubChem SID: 24889414
• MDL Number: MFCD20921517
• CAS Number: 84537-86-0

PROPERTIES

• Empirical Formula (Hill Notation): C32H25N7Ru · 2 PF6
• Product Line: BioReagent
• Suitability: suitable for fluorescence
• Fluorescence: λex 458 nm; λem 612 nm in 0.1 M Tris pH 7.0
• Personal Protective Equipment: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• Storage Temperature: -20°C
• German water hazard class: 3

DETAILS

Description (English)

Application
Used for photoinduced intramolecular electron transfer in peptide-bridged molecules.
Features and Benefits
Sigma-Aldrich offers a series of reactive dyes based on ruthenium complexes for use in fluorescence polarization assays and fluorescence lifetime imaging applications. They have been designed specifically to have long fluorescence lifetimes. These dyes were first synthesized in the labs of Dr. Joseph Lakowicz at the Center for Fluorescence Microscopy. These laser-excitable metal ligand complexes have fluorescence lifetimes near 500 nanoseconds.These dyes can be used to label amines on biomolecules under mild conditions. Advantages of these dyes as protein labels include high photostability, good water solubility, a lack of dye-dye interactions and large Stokes’ shifts.In addition, the fluorescence signal of long-lived fluorophores can be gated to eliminate the emission from short-lifetime fluorophores and autofluorescence from cells and biomolecules to further improve sensitivity.
Other Notes
Photoinduced intramolecular electron transfer in peptide-bridged molecules1,2

Description (简体中文)

Application
用于肽桥分子中的光诱导分子内电子转移。
Features and Benefits
Sigma-Aldrich 提供一系列基于钌络合物的活性染料，用于荧光偏振检测和荧光寿命成像。这些染料的设计独特，具有较长的荧光寿命。它们首先在荧光显微中心 Dr. Joseph Lakowicz 的实验室中合成。这些激光激发的金属配体络合物具有接近 500 纳秒的荧光寿命。 这些染料可用于在温和条件下标记生物分子上的胺。作为蛋白质标签，其优点在于良好的光稳定性和水溶性、染料之间无相互作用，并且斯托克斯位移较大。 另外，可以选通长寿命荧光团的荧光信号，从而消除短寿命荧光团发光，并且消除细胞和生物分子的自发荧光，从而进一步提高灵敏度。
Other Notes
肽桥分子中的光诱导分子内电子转移。1,2